| pubmed-article:19108884 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:19108884 | lifeskim:mentions | umls-concept:C1123023 | lld:lifeskim |
| pubmed-article:19108884 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:19108884 | lifeskim:mentions | umls-concept:C0010453 | lld:lifeskim |
| pubmed-article:19108884 | lifeskim:mentions | umls-concept:C0022567 | lld:lifeskim |
| pubmed-article:19108884 | lifeskim:mentions | umls-concept:C0016030 | lld:lifeskim |
| pubmed-article:19108884 | lifeskim:mentions | umls-concept:C1708099 | lld:lifeskim |
| pubmed-article:19108884 | lifeskim:mentions | umls-concept:C2349966 | lld:lifeskim |
| pubmed-article:19108884 | lifeskim:mentions | umls-concept:C0450363 | lld:lifeskim |
| pubmed-article:19108884 | pubmed:issue | 8 | lld:pubmed |
| pubmed-article:19108884 | pubmed:dateCreated | 2009-1-23 | lld:pubmed |
| pubmed-article:19108884 | pubmed:abstractText | We present a method to create multi-layered engineered tissue composites consisting of human skin fibroblasts and keratinocytes which mimic skin layers. Three-dimensional (3D) freeform fabrication (FF) technique, based on direct cell dispensing, was implemented using a robotic platform that prints collagen hydrogel precursor, fibroblasts and keratinocytes. A printed layer of cell-containing collagen was crosslinked by coating the layer with nebulized aqueous sodium bicarbonate. The process was repeated in layer-by-layer fashion on a planar tissue culture dish, resulting in two distinct cell layers of inner fibroblasts and outer keratinocytes. In order to demonstrate the ability to print and culture multi-layered cell-hydrogel composites on a non-planar surface for potential applications including skin wound repair, the technique was tested on a poly(dimethylsiloxane) (PDMS) mold with 3D surface contours as a target substrate. Highly viable proliferation of each cell layer was observed on both planar and non-planar surfaces. Our results suggest that organotypic skin tissue culture is feasible using on-demand cell printing technique with future potential application in creating skin grafts tailored for wound shape or artificial tissue assay for disease modeling and drug testing. | lld:pubmed |
| pubmed-article:19108884 | pubmed:language | eng | lld:pubmed |
| pubmed-article:19108884 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19108884 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:19108884 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19108884 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19108884 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19108884 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19108884 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:19108884 | pubmed:month | Mar | lld:pubmed |
| pubmed-article:19108884 | pubmed:issn | 1878-5905 | lld:pubmed |
| pubmed-article:19108884 | pubmed:author | pubmed-author:LeeJong-HwanJ... | lld:pubmed |
| pubmed-article:19108884 | pubmed:author | pubmed-author:YooSeung-Schi... | lld:pubmed |
| pubmed-article:19108884 | pubmed:author | pubmed-author:ParkJe-KyunJK | lld:pubmed |
| pubmed-article:19108884 | pubmed:author | pubmed-author:FischerKriszt... | lld:pubmed |
| pubmed-article:19108884 | pubmed:author | pubmed-author:LeeWonhyeW | lld:pubmed |
| pubmed-article:19108884 | pubmed:author | pubmed-author:DebasitisJaso... | lld:pubmed |
| pubmed-article:19108884 | pubmed:author | pubmed-author:LeeVivian... | lld:pubmed |
| pubmed-article:19108884 | pubmed:author | pubmed-author:EdminsterKarl... | lld:pubmed |
| pubmed-article:19108884 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:19108884 | pubmed:volume | 30 | lld:pubmed |
| pubmed-article:19108884 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:19108884 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:19108884 | pubmed:pagination | 1587-95 | lld:pubmed |
| pubmed-article:19108884 | pubmed:meshHeading | pubmed-meshheading:19108884... | lld:pubmed |
| pubmed-article:19108884 | pubmed:meshHeading | pubmed-meshheading:19108884... | lld:pubmed |
| pubmed-article:19108884 | pubmed:meshHeading | pubmed-meshheading:19108884... | lld:pubmed |
| pubmed-article:19108884 | pubmed:meshHeading | pubmed-meshheading:19108884... | lld:pubmed |
| pubmed-article:19108884 | pubmed:meshHeading | pubmed-meshheading:19108884... | lld:pubmed |
| pubmed-article:19108884 | pubmed:meshHeading | pubmed-meshheading:19108884... | lld:pubmed |
| pubmed-article:19108884 | pubmed:meshHeading | pubmed-meshheading:19108884... | lld:pubmed |
| pubmed-article:19108884 | pubmed:meshHeading | pubmed-meshheading:19108884... | lld:pubmed |
| pubmed-article:19108884 | pubmed:meshHeading | pubmed-meshheading:19108884... | lld:pubmed |
| pubmed-article:19108884 | pubmed:meshHeading | pubmed-meshheading:19108884... | lld:pubmed |
| pubmed-article:19108884 | pubmed:meshHeading | pubmed-meshheading:19108884... | lld:pubmed |
| pubmed-article:19108884 | pubmed:year | 2009 | lld:pubmed |
| pubmed-article:19108884 | pubmed:articleTitle | Multi-layered culture of human skin fibroblasts and keratinocytes through three-dimensional freeform fabrication. | lld:pubmed |
| pubmed-article:19108884 | pubmed:affiliation | Department of Radiology, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA. | lld:pubmed |
| pubmed-article:19108884 | pubmed:publicationType | Journal Article | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:19108884 | lld:pubmed |
