| pubmed-article:19094061 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:19094061 | lifeskim:mentions | umls-concept:C0027882 | lld:lifeskim |
| pubmed-article:19094061 | lifeskim:mentions | umls-concept:C0007776 | lld:lifeskim |
| pubmed-article:19094061 | lifeskim:mentions | umls-concept:C0022655 | lld:lifeskim |
| pubmed-article:19094061 | lifeskim:mentions | umls-concept:C0001613 | lld:lifeskim |
| pubmed-article:19094061 | lifeskim:mentions | umls-concept:C0006685 | lld:lifeskim |
| pubmed-article:19094061 | lifeskim:mentions | umls-concept:C0043791 | lld:lifeskim |
| pubmed-article:19094061 | lifeskim:mentions | umls-concept:C1515877 | lld:lifeskim |
| pubmed-article:19094061 | lifeskim:mentions | umls-concept:C0243071 | lld:lifeskim |
| pubmed-article:19094061 | lifeskim:mentions | umls-concept:C1879547 | lld:lifeskim |
| pubmed-article:19094061 | lifeskim:mentions | umls-concept:C0871161 | lld:lifeskim |
| pubmed-article:19094061 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:19094061 | pubmed:dateCreated | 2008-12-19 | lld:pubmed |
| pubmed-article:19094061 | pubmed:abstractText | The lipid diacylglycerol (DAG) analogue 1-oleoyl-2-acetyl-sn-glycerol (OAG) was used to verify the existence of DAG-sensitive channels in cortical neurons dissociated from E13 mouse embryos. Calcium imaging experiments showed that OAG increased the cytosolic concentration of Ca(2+) ([Ca(2+)]i) in nearly 35% of the KCl-responsive cells. These Ca(2+) responses disappeared in a Ca(2+)-free medium supplemented with EGTA. Mn(2+) quench experiments showed that OAG activated Ca(2+)-conducting channels that were also permeant to Ba(2+). The OAG-induced Ca(2+) responses were unaffected by nifedipine or omega-conotoxin GVIA (Sigma-Aldrich, Saint-Quentin Fallavier, France) but blocked by 1-[beta-(3-(4-Methoxyphenyl)propoxy)-4-methoxyphenethyl]-1H-imidazole hydrochloride (SKF)-96365 and Gd(3+). Replacing Na(+) ions with N-methyl-D-glucamine diminished the amplitude of the OAG-induced Ca(2+) responses showing that the Ca(2+) entry was mediated via Na(+)-dependent and Na(+)-independent mechanisms. Experiments carried out with the fluorescent Na(+) indicator CoroNa Green showed that OAG elevated [Na(+)]i. Like OAG, the DAG lipase inhibitor RHC80267 increased [Ca(2+)]i but not the protein kinase C activator phorbol 12-myristate 13-acetate. Moreover, the OAG-induced Ca(2+) responses were not regulated by protein kinase C activation or inhibition but they were augmented by flufenamic acid which increases currents through C-type transient receptor potential protein family (TRPC) 6 channels. In addition, application of hyperforin, a specific activator of TRPC6 channels, elevated [Ca(2+)]i. Whole-cell patch-clamp recordings showed that hyperforin activated non-selective cation channels. They were blocked by SKF-96365 but potentiated by flufenamic acid. Altogether, our data show the presence of hyperforin- and OAG-sensitive Ca(2+)-permeable channels displaying TRPC6-like properties. This is the first report revealing the existence of second messenger-operated channels in cortical neurons. | lld:pubmed |
| pubmed-article:19094061 | pubmed:language | eng | lld:pubmed |
| pubmed-article:19094061 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19094061 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:19094061 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19094061 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:19094061 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:19094061 | pubmed:month | Jan | lld:pubmed |
| pubmed-article:19094061 | pubmed:issn | 1471-4159 | lld:pubmed |
| pubmed-article:19094061 | pubmed:author | pubmed-author:BrinkmeierHei... | lld:pubmed |
| pubmed-article:19094061 | pubmed:author | pubmed-author:Kunert-KeilCh... | lld:pubmed |
| pubmed-article:19094061 | pubmed:author | pubmed-author:LuckeSilkeS | lld:pubmed |
| pubmed-article:19094061 | pubmed:author | pubmed-author:BouronAlexand... | lld:pubmed |
| pubmed-article:19094061 | pubmed:author | pubmed-author:TuPengP | lld:pubmed |
| pubmed-article:19094061 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:19094061 | pubmed:volume | 108 | lld:pubmed |
| pubmed-article:19094061 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:19094061 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:19094061 | pubmed:pagination | 126-38 | lld:pubmed |
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| pubmed-article:19094061 | pubmed:year | 2009 | lld:pubmed |
| pubmed-article:19094061 | pubmed:articleTitle | Diacylglycerol analogues activate second messenger-operated calcium channels exhibiting TRPC-like properties in cortical neurons. | lld:pubmed |
| pubmed-article:19094061 | pubmed:affiliation | CNRS UMR 5249, Grenoble, France. | lld:pubmed |
| pubmed-article:19094061 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:19094061 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| entrez-gene:22068 | entrezgene:pubmed | pubmed-article:19094061 | lld:entrezgene |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:19094061 | lld:pubmed |
