| pubmed-article:19056509 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:19056509 | lifeskim:mentions | umls-concept:C0019704 | lld:lifeskim |
| pubmed-article:19056509 | lifeskim:mentions | umls-concept:C0007634 | lld:lifeskim |
| pubmed-article:19056509 | lifeskim:mentions | umls-concept:C0443288 | lld:lifeskim |
| pubmed-article:19056509 | lifeskim:mentions | umls-concept:C0231180 | lld:lifeskim |
| pubmed-article:19056509 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:19056509 | pubmed:dateCreated | 2009-3-10 | lld:pubmed |
| pubmed-article:19056509 | pubmed:databankReference | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19056509 | pubmed:databankReference | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19056509 | pubmed:databankReference | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19056509 | pubmed:abstractText | We have recently generated a monkey cell-tropic virus termed NL-DT5R from an HIV-1 NL4-3 clone and demonstrated that both cyclophilin A (CypA)-binding loop in Gag-capsid (CA) and Vif are responsible for the species-restriction of HIV-1. In this study, we constructed 16 CypA-binding loop mutants from the HIV-1-derivative NL-DT5R, and analyzed them biologically and biochemically. The mutants displayed various multi-cycle infection potencies in cynomolgus monkey (CyM) HSC-F cells, but none of them grew significantly better than NL-DT5R. Consistently, any of the HIV-1 variants examined here did not effectively counter CyM TRIM5alpha as judged by single-cycle infectivity assays. Assessment of their single-cycle infectivity in simian and CyM TRIM5alpha-expressing feline cells in the presence of cyclosporin A (CsA) showed that intervention of CypA-CA interaction did not restore full NL-DT5R infectivity, while CsA increased infectivity of DT5R/4-3 carrying the sequence of NL4-3 CypA-binding loop up to the NL-DT5R level. Almost similar data were obtained in the experiments utilizing CypA-targeting siRNA. Together with our previous results regarding NL-DT5R, these data suggested that evasion from CypA- and APOBEC-mediated restrictions is still insufficient for HIV-1 to completely overcome the species barrier. | lld:pubmed |
| pubmed-article:19056509 | pubmed:language | eng | lld:pubmed |
| pubmed-article:19056509 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19056509 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:19056509 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19056509 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19056509 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19056509 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:19056509 | pubmed:month | Feb | lld:pubmed |
| pubmed-article:19056509 | pubmed:issn | 1769-714X | lld:pubmed |
| pubmed-article:19056509 | pubmed:author | pubmed-author:AdachiAkioA | lld:pubmed |
| pubmed-article:19056509 | pubmed:author | pubmed-author:NomaguchiMasa... | lld:pubmed |
| pubmed-article:19056509 | pubmed:author | pubmed-author:KamadaKazuyaK | lld:pubmed |
| pubmed-article:19056509 | pubmed:author | pubmed-author:YamashitaTomo... | lld:pubmed |
| pubmed-article:19056509 | pubmed:author | pubmed-author:HatchoKazukiK | lld:pubmed |
| pubmed-article:19056509 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:19056509 | pubmed:volume | 11 | lld:pubmed |
| pubmed-article:19056509 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:19056509 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:19056509 | pubmed:pagination | 164-71 | lld:pubmed |
| pubmed-article:19056509 | pubmed:dateRevised | 2009-6-18 | lld:pubmed |
| pubmed-article:19056509 | pubmed:meshHeading | pubmed-meshheading:19056509... | lld:pubmed |
| pubmed-article:19056509 | pubmed:meshHeading | pubmed-meshheading:19056509... | lld:pubmed |
| pubmed-article:19056509 | pubmed:meshHeading | pubmed-meshheading:19056509... | lld:pubmed |
| pubmed-article:19056509 | pubmed:meshHeading | pubmed-meshheading:19056509... | lld:pubmed |
| pubmed-article:19056509 | pubmed:meshHeading | pubmed-meshheading:19056509... | lld:pubmed |
| pubmed-article:19056509 | pubmed:meshHeading | pubmed-meshheading:19056509... | lld:pubmed |
| pubmed-article:19056509 | pubmed:meshHeading | pubmed-meshheading:19056509... | lld:pubmed |
| pubmed-article:19056509 | pubmed:meshHeading | pubmed-meshheading:19056509... | lld:pubmed |
| pubmed-article:19056509 | pubmed:meshHeading | pubmed-meshheading:19056509... | lld:pubmed |
| pubmed-article:19056509 | pubmed:meshHeading | pubmed-meshheading:19056509... | lld:pubmed |
| pubmed-article:19056509 | pubmed:year | 2009 | lld:pubmed |
| pubmed-article:19056509 | pubmed:articleTitle | Evasion from CypA- and APOBEC-mediated restrictions is insufficient for HIV-1 to efficiently grow in simian cells. | lld:pubmed |
| pubmed-article:19056509 | pubmed:affiliation | Department of Virology, Institute of Health Biosciences, The University of Tokushima Graduate School, Tokushima 770-8503, Japan. | lld:pubmed |
| pubmed-article:19056509 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:19056509 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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