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pubmed-article:1902840pubmed:abstractTextA differential kinetic study of 13CO2 enrichment of breath after the intake of specific 13C-labelled substrates and co-administration of a drug allows the drug's ability for enzyme induction to be evaluated in vivo. A method and a gas chromatograph-isotope ratio mass spectrometer device for on-line measurements of 13CO2 enrichment in the breath of small animals are described. This system allows on-line breath sample collection from a metabolic cage, purification by gas chromatography, determination of CO2 by thermal conductivity detection and measurement of 13CO2 enrichment by isotope ratio mass spectrometry. Two protocols for phenobarbital-inducible P450 and 3-methylcholanthrene-inducible P1-450 isoenzymes are described.lld:pubmed
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pubmed-article:1902840pubmed:dateRevised2006-11-15lld:pubmed
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pubmed-article:1902840pubmed:articleTitleOn-line measurements of 13C enrichments in rat breath. Non-invasive method for in vivo study of drug enzymatic induction.lld:pubmed
pubmed-article:1902840pubmed:affiliationL.E.A.C.M., Laboratoire d'Etudes Analytiques et Cinétiques du Médicament, Institut des Sciences Pharmaceutiques et Biologiques de Lyon, France.lld:pubmed
pubmed-article:1902840pubmed:publicationTypeJournal Articlelld:pubmed