| pubmed-article:19019086 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:19019086 | lifeskim:mentions | umls-concept:C1956385 | lld:lifeskim |
| pubmed-article:19019086 | lifeskim:mentions | umls-concept:C0012854 | lld:lifeskim |
| pubmed-article:19019086 | lifeskim:mentions | umls-concept:C0002199 | lld:lifeskim |
| pubmed-article:19019086 | lifeskim:mentions | umls-concept:C0085862 | lld:lifeskim |
| pubmed-article:19019086 | lifeskim:mentions | umls-concept:C1299583 | lld:lifeskim |
| pubmed-article:19019086 | lifeskim:mentions | umls-concept:C1428114 | lld:lifeskim |
| pubmed-article:19019086 | lifeskim:mentions | umls-concept:C1519249 | lld:lifeskim |
| pubmed-article:19019086 | lifeskim:mentions | umls-concept:C0851285 | lld:lifeskim |
| pubmed-article:19019086 | lifeskim:mentions | umls-concept:C0205263 | lld:lifeskim |
| pubmed-article:19019086 | lifeskim:mentions | umls-concept:C1879547 | lld:lifeskim |
| pubmed-article:19019086 | lifeskim:mentions | umls-concept:C1608386 | lld:lifeskim |
| pubmed-article:19019086 | lifeskim:mentions | umls-concept:C1549571 | lld:lifeskim |
| pubmed-article:19019086 | lifeskim:mentions | umls-concept:C0599124 | lld:lifeskim |
| pubmed-article:19019086 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:19019086 | pubmed:dateCreated | 2009-1-7 | lld:pubmed |
| pubmed-article:19019086 | pubmed:abstractText | Single-stranded versus multimeric phosphorothioate-modified CpG oligodeoxynucleotides (ODNs) undergo differential endosomal trafficking upon uptake into plasmacytoid dendritic cells (pDCs), correlating with Toll-like receptor-9-dependent pDC maturation/activation (single-stranded B-type CpG ODN) or interferon-alpha (IFN-alpha) induction (multimeric A-type CpG ODN), respectively. As was recently shown, IFN-alpha production, other than by CpG ODNs, can also be induced in a sequence-independent manner by phosphodiester (PD) ODNs multimerized by 3' poly-guanosine (poly-G) tails. We investigate here the type of endosomal trafficking responsible for IFN-alpha induction by natural PD ODN ligands. We show that 3' extension with poly-G tails leads to multimerization of single-stranded PD ODNs and to enhanced cellular uptake into pDCs. While monomeric PD ODNs, which induce CpG-dependent Toll-like receptor-9 stimulation and pDC maturation/activation, localized to late endosomal/lysosomal compartments, the poly-G multimerized PD ODNs, which induce CpG-independent IFN-alpha production, located to vesicles with a distinct, 'early' endosomal phenotype. We conclude that poly-G-mediated multimerization of natural PD ODNs allows for sequence-independent, Toll-like receptor-9-dependent IFN-alpha induction in pDCs by combining three distinct effects: relative protection of sensitive PD ODNs from extracellular and intracellular DNase degradation, enhanced cellular uptake and preferential early endosomal compartmentation. | lld:pubmed |
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| pubmed-article:19019086 | pubmed:language | eng | lld:pubmed |
| pubmed-article:19019086 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19019086 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:19019086 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:19019086 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:19019086 | pubmed:month | Feb | lld:pubmed |
| pubmed-article:19019086 | pubmed:issn | 1365-2567 | lld:pubmed |
| pubmed-article:19019086 | pubmed:author | pubmed-author:WagnerHermann... | lld:pubmed |
| pubmed-article:19019086 | pubmed:author | pubmed-author:SchmitzFrankF | lld:pubmed |
| pubmed-article:19019086 | pubmed:author | pubmed-author:HeitAntjeA | lld:pubmed |
| pubmed-article:19019086 | pubmed:author | pubmed-author:HaasTobiasT | lld:pubmed |
| pubmed-article:19019086 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:19019086 | pubmed:volume | 126 | lld:pubmed |
| pubmed-article:19019086 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:19019086 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:19019086 | pubmed:pagination | 290-8 | lld:pubmed |
| pubmed-article:19019086 | pubmed:dateRevised | 2010-9-23 | lld:pubmed |
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| pubmed-article:19019086 | pubmed:meshHeading | pubmed-meshheading:19019086... | lld:pubmed |
| pubmed-article:19019086 | pubmed:year | 2009 | lld:pubmed |
| pubmed-article:19019086 | pubmed:articleTitle | Sequence independent interferon-alpha induction by multimerized phosphodiester DNA depends on spatial regulation of Toll-like receptor-9 activation in plasmacytoid dendritic cells. | lld:pubmed |
| pubmed-article:19019086 | pubmed:affiliation | Institut für Medizinische Mikrobiologie, Immunologie und Hygiene, Technische Universität München, Trogerstrasse, Munich, Germany. | lld:pubmed |
| pubmed-article:19019086 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:19019086 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| entrez-gene:81897 | entrezgene:pubmed | pubmed-article:19019086 | lld:entrezgene |
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