| pubmed-article:19003004 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:19003004 | lifeskim:mentions | umls-concept:C0025519 | lld:lifeskim |
| pubmed-article:19003004 | lifeskim:mentions | umls-concept:C0007577 | lld:lifeskim |
| pubmed-article:19003004 | lifeskim:mentions | umls-concept:C0302523 | lld:lifeskim |
| pubmed-article:19003004 | lifeskim:mentions | umls-concept:C0700325 | lld:lifeskim |
| pubmed-article:19003004 | lifeskim:mentions | umls-concept:C0205148 | lld:lifeskim |
| pubmed-article:19003004 | lifeskim:mentions | umls-concept:C0260249 | lld:lifeskim |
| pubmed-article:19003004 | lifeskim:mentions | umls-concept:C0444498 | lld:lifeskim |
| pubmed-article:19003004 | pubmed:issue | 2-3 | lld:pubmed |
| pubmed-article:19003004 | pubmed:dateCreated | 2008-11-12 | lld:pubmed |
| pubmed-article:19003004 | pubmed:abstractText | In this study, we report on an in situ monitoring system of living cultured cells using infrared absorption spectroscopy in the geometry of multiple internal reflections (MIR-IRAS). In order to observe living cultured cells, the temperature in the sample chamber of a FT-IR spectrometer was maintained at 37 degrees C and a humidified gas mixture containing 5% CO(2) was introduced into the sample chamber. Human breast cell line MCF-7 cultured on Si MIR prisms were placed in the sample chamber and infrared spectra of MCF-7 cells were collected for 5 h. It was found that the adhesion and metabolism of MCF-7 cells could be monitored by the absorption intensity of amide-II protein band (1,545 cm(-1)) and also by the absorption intensities of CH( x ) bands (2,700-3,100 cm(-1)). These results suggest that our system is useful for a nondestructive and non-label monitoring of cell viability. Our method based on infrared absorption spectroscopy has a potential for bioscreening application. | lld:pubmed |
| pubmed-article:19003004 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19003004 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19003004 | pubmed:language | eng | lld:pubmed |
| pubmed-article:19003004 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19003004 | pubmed:status | PubMed-not-MEDLINE | lld:pubmed |
| pubmed-article:19003004 | pubmed:month | Dec | lld:pubmed |
| pubmed-article:19003004 | pubmed:issn | 0920-9069 | lld:pubmed |
| pubmed-article:19003004 | pubmed:author | pubmed-author:KimuraYasuoY | lld:pubmed |
| pubmed-article:19003004 | pubmed:author | pubmed-author:IsodaHirokoH | lld:pubmed |
| pubmed-article:19003004 | pubmed:author | pubmed-author:HiranoAyumiA | lld:pubmed |
| pubmed-article:19003004 | pubmed:author | pubmed-author:YamadaParidaP | lld:pubmed |
| pubmed-article:19003004 | pubmed:author | pubmed-author:NiwanoMichioM | lld:pubmed |
| pubmed-article:19003004 | pubmed:author | pubmed-author:MiyamotoKo-Ic... | lld:pubmed |
| pubmed-article:19003004 | pubmed:author | pubmed-author:YamaguchiRyo-... | lld:pubmed |
| pubmed-article:19003004 | pubmed:author | pubmed-author:MutoTakamiT | lld:pubmed |
| pubmed-article:19003004 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:19003004 | pubmed:volume | 55 | lld:pubmed |
| pubmed-article:19003004 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:19003004 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:19003004 | pubmed:pagination | 143-9 | lld:pubmed |
| pubmed-article:19003004 | pubmed:year | 2007 | lld:pubmed |
| pubmed-article:19003004 | pubmed:articleTitle | In situ observation of a cell adhesion and metabolism using surface infrared spectroscopy. | lld:pubmed |
| pubmed-article:19003004 | pubmed:affiliation | Department of Electronic Engineering, Graduate School of Engineering, Tohoku University, 6-6-05 Aza-Aoba, Aramaki, Aoba-ku, Sendai, Miyagi, 980-8579, Japan. | lld:pubmed |
| pubmed-article:19003004 | pubmed:publicationType | Journal Article | lld:pubmed |
