| pubmed-article:1886770 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:1886770 | lifeskim:mentions | umls-concept:C0017337 | lld:lifeskim |
| pubmed-article:1886770 | lifeskim:mentions | umls-concept:C0204727 | lld:lifeskim |
| pubmed-article:1886770 | lifeskim:mentions | umls-concept:C0205409 | lld:lifeskim |
| pubmed-article:1886770 | lifeskim:mentions | umls-concept:C0162326 | lld:lifeskim |
| pubmed-article:1886770 | lifeskim:mentions | umls-concept:C0178499 | lld:lifeskim |
| pubmed-article:1886770 | lifeskim:mentions | umls-concept:C0332256 | lld:lifeskim |
| pubmed-article:1886770 | lifeskim:mentions | umls-concept:C1704788 | lld:lifeskim |
| pubmed-article:1886770 | lifeskim:mentions | umls-concept:C0684192 | lld:lifeskim |
| pubmed-article:1886770 | pubmed:issue | 16 | lld:pubmed |
| pubmed-article:1886770 | pubmed:dateCreated | 1991-10-4 | lld:pubmed |
| pubmed-article:1886770 | pubmed:abstractText | We have established a sensitive, monoclonal antibody (Mab)-based procedure permitting the selective enrichment of sequences containing the miscoding alkylation product O6-ethylguanine (O6-EtGua) from mammalian DNA. H5 rat hepatoma cells were reacted with the N-nitroso carcinogen N-ethyl-N-nitrosourea in vitro, to give overall levels of greater than or equal to 25 O6-EtGua residues per diploid genome (corresponding to O6-EtGua/guanine molar ratios of greater than or equal to 10(-8). For analysis, enzymatically restricted DNA from these cells is incubated with an antibody specific for O6-ethyl-2'-deoxyguanosine, the resulting Mab-DNA complexes are separated from (O6-EtGua)-free fragments by filtration through a nitrocellulose (NC) membrane, and the DNA is recovered from the filter-bound complexes quantitatively. The efficiency of Mab binding to DNA fragments containing O6-EtGua is constant over a range of O6-EtGua/guanine molar ratios between 10(-5) and 10(-8). (O6-EtGua)-containing restriction fragments encompassing known gene sequences (e.g., the immunoglobulin E heavy chain gene of H5 rat hepatoma cells used as a model in this study) are subsequently amplified by PCR and quantified by slot-blot hybridisation. The content and distribution of a specific carcinogen-DNA adduct in defined sequences of genomic DNA can thus be analyzed as well as the kinetics of intragenomic (toposelective) repair of any DNA lesion for which a suitable Mab is available. | lld:pubmed |
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| pubmed-article:1886770 | pubmed:language | eng | lld:pubmed |
| pubmed-article:1886770 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1886770 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:1886770 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:1886770 | pubmed:month | Aug | lld:pubmed |
| pubmed-article:1886770 | pubmed:issn | 0305-1048 | lld:pubmed |
| pubmed-article:1886770 | pubmed:author | pubmed-author:RajewskyM FMF | lld:pubmed |
| pubmed-article:1886770 | pubmed:author | pubmed-author:ThomaleJJ | lld:pubmed |
| pubmed-article:1886770 | pubmed:author | pubmed-author:Nikitin AYu | lld:pubmed |
| pubmed-article:1886770 | pubmed:author | pubmed-author:HochleitnerKK | lld:pubmed |
| pubmed-article:1886770 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:1886770 | pubmed:day | 25 | lld:pubmed |
| pubmed-article:1886770 | pubmed:volume | 19 | lld:pubmed |
| pubmed-article:1886770 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:1886770 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:1886770 | pubmed:pagination | 4467-72 | lld:pubmed |
| pubmed-article:1886770 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:1886770 | pubmed:year | 1991 | lld:pubmed |
| pubmed-article:1886770 | pubmed:articleTitle | Monoclonal antibody-based, selective isolation of DNA fragments containing an alkylated base to be quantified in defined gene sequences. | lld:pubmed |
| pubmed-article:1886770 | pubmed:affiliation | Institute of Cell Biology (Cancer Research), University of Essen Medical School, FRG. | lld:pubmed |
| pubmed-article:1886770 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:1886770 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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