| pubmed-article:18835854 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:18835854 | lifeskim:mentions | umls-concept:C0024662 | lld:lifeskim |
| pubmed-article:18835854 | lifeskim:mentions | umls-concept:C0020792 | lld:lifeskim |
| pubmed-article:18835854 | lifeskim:mentions | umls-concept:C0013879 | lld:lifeskim |
| pubmed-article:18835854 | pubmed:issue | 19 | lld:pubmed |
| pubmed-article:18835854 | pubmed:dateCreated | 2008-11-4 | lld:pubmed |
| pubmed-article:18835854 | pubmed:abstractText | Mouse mammary tumor virus (MMTV) has previously been shown to encode a functional homolog of the human immunodeficiency virus-1 (HIV-1) nuclear export protein Rev, termed Rem. Here, we show that deletion of the rem gene from a MMTV molecular clone interfered with the nucleo-cytoplasmic transport of genomic length viral mRNA and resulted in a loss of viral capsid (Gag) protein production. Interestingly, nuclear export of single-spliced env mRNA was only moderately affected, suggesting that this transcript is, at least to some extent, transported via a distinct, Rem-independent export mechanism. To identify and characterize a cis-acting RNA element required for Rem responsiveness (RmRE), extensive computational and functional analyses were performed. By these means a region of 490 nt corresponding to positions nt 8517-nt 9006 in the MMTV reference strain was identified as RmRE. Deletion of this fragment, which spans the env-U3 junction region, abolished Gag expression. Furthermore, insertion of this sequence into a heterologous HIV-1-based reporter construct restored, in the presence of Rem, HIV-1 Gag expression to levels determined for the Rev/RRE export system. These results clearly demonstrate that the identified region, whose geometry resembles that of other retroviral-responsive elements, is capable to functionally substitute, in the presence of Rem, for Rev/RRE and thus provide unequivocal evidence that MMTV is a complex retrovirus. | lld:pubmed |
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| pubmed-article:18835854 | pubmed:language | eng | lld:pubmed |
| pubmed-article:18835854 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18835854 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:18835854 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:18835854 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:18835854 | pubmed:month | Nov | lld:pubmed |
| pubmed-article:18835854 | pubmed:issn | 1362-4962 | lld:pubmed |
| pubmed-article:18835854 | pubmed:author | pubmed-author:SalmonsBrianB | lld:pubmed |
| pubmed-article:18835854 | pubmed:author | pubmed-author:GünzburgWalte... | lld:pubmed |
| pubmed-article:18835854 | pubmed:author | pubmed-author:IndikStanisla... | lld:pubmed |
| pubmed-article:18835854 | pubmed:author | pubmed-author:MüllnerMatthi... | lld:pubmed |
| pubmed-article:18835854 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:18835854 | pubmed:volume | 36 | lld:pubmed |
| pubmed-article:18835854 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:18835854 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:18835854 | pubmed:pagination | 6284-94 | lld:pubmed |
| pubmed-article:18835854 | pubmed:dateRevised | 2011-4-6 | lld:pubmed |
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| pubmed-article:18835854 | pubmed:year | 2008 | lld:pubmed |
| pubmed-article:18835854 | pubmed:articleTitle | Identification of the Rem-responsive element of mouse mammary tumor virus. | lld:pubmed |
| pubmed-article:18835854 | pubmed:affiliation | Department of Pathobiology, Institute of Virology, University of Veterinary Medicine Vienna, Austria. | lld:pubmed |
| pubmed-article:18835854 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:18835854 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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