| pubmed-article:18656369 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:18656369 | lifeskim:mentions | umls-concept:C0678614 | lld:lifeskim |
| pubmed-article:18656369 | lifeskim:mentions | umls-concept:C1414387 | lld:lifeskim |
| pubmed-article:18656369 | lifeskim:mentions | umls-concept:C0016315 | lld:lifeskim |
| pubmed-article:18656369 | lifeskim:mentions | umls-concept:C1510438 | lld:lifeskim |
| pubmed-article:18656369 | pubmed:issue | 16 | lld:pubmed |
| pubmed-article:18656369 | pubmed:dateCreated | 2008-8-18 | lld:pubmed |
| pubmed-article:18656369 | pubmed:abstractText | We report herein the development of an efficient fluorescence assay for serine/threonine kinases using a peptide array. Our approach is based on chemical reactions specific to phosphoserine and phosphothreonine residues, that is, base-mediated beta-elimination of the phosphate group and subsequent Michael addition of a thiol-containing fluorescent reagent. This procedure enables the covalent introduction of a fluorescent moiety into the phosphorylated peptide. Novel fluorescent reagents were designed for this purpose and synthesized. With these reagents, protein kinase A (PKA) and Akt-1 activities were readily detected. Our method can also be used to measure the activity of kinase inhibitors. This assay is expected to be widely applicable in kinase research. | lld:pubmed |
| pubmed-article:18656369 | pubmed:language | eng | lld:pubmed |
| pubmed-article:18656369 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18656369 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:18656369 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18656369 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18656369 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18656369 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18656369 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18656369 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18656369 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18656369 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:18656369 | pubmed:month | Aug | lld:pubmed |
| pubmed-article:18656369 | pubmed:issn | 1464-3391 | lld:pubmed |
| pubmed-article:18656369 | pubmed:author | pubmed-author:UmezawaNaokiN | lld:pubmed |
| pubmed-article:18656369 | pubmed:author | pubmed-author:KatoNobukiN | lld:pubmed |
| pubmed-article:18656369 | pubmed:author | pubmed-author:HiguchiTsuneh... | lld:pubmed |
| pubmed-article:18656369 | pubmed:author | pubmed-author:AkitaShojiS | lld:pubmed |
| pubmed-article:18656369 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:18656369 | pubmed:day | 15 | lld:pubmed |
| pubmed-article:18656369 | pubmed:volume | 16 | lld:pubmed |
| pubmed-article:18656369 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:18656369 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:18656369 | pubmed:pagination | 7788-94 | lld:pubmed |
| pubmed-article:18656369 | pubmed:meshHeading | pubmed-meshheading:18656369... | lld:pubmed |
| pubmed-article:18656369 | pubmed:meshHeading | pubmed-meshheading:18656369... | lld:pubmed |
| pubmed-article:18656369 | pubmed:meshHeading | pubmed-meshheading:18656369... | lld:pubmed |
| pubmed-article:18656369 | pubmed:meshHeading | pubmed-meshheading:18656369... | lld:pubmed |
| pubmed-article:18656369 | pubmed:meshHeading | pubmed-meshheading:18656369... | lld:pubmed |
| pubmed-article:18656369 | pubmed:meshHeading | pubmed-meshheading:18656369... | lld:pubmed |
| pubmed-article:18656369 | pubmed:meshHeading | pubmed-meshheading:18656369... | lld:pubmed |
| pubmed-article:18656369 | pubmed:meshHeading | pubmed-meshheading:18656369... | lld:pubmed |
| pubmed-article:18656369 | pubmed:meshHeading | pubmed-meshheading:18656369... | lld:pubmed |
| pubmed-article:18656369 | pubmed:meshHeading | pubmed-meshheading:18656369... | lld:pubmed |
| pubmed-article:18656369 | pubmed:meshHeading | pubmed-meshheading:18656369... | lld:pubmed |
| pubmed-article:18656369 | pubmed:year | 2008 | lld:pubmed |
| pubmed-article:18656369 | pubmed:articleTitle | Array-based fluorescence assay for serine/threonine kinases using specific chemical reaction. | lld:pubmed |
| pubmed-article:18656369 | pubmed:affiliation | Graduate School of Pharmaceutical Sciences, Nagoya City University, 3-1 Tanabe-dori, Mizuho-ku, Nagoya, Aichi 467-8603, Japan. | lld:pubmed |
| pubmed-article:18656369 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:18656369 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | http://linkedlifedata.com/r... | pubmed-article:18656369 | lld:chembl |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:18656369 | lld:pubmed |
