| pubmed-article:1863470 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:1863470 | lifeskim:mentions | umls-concept:C0019682 | lld:lifeskim |
| pubmed-article:1863470 | lifeskim:mentions | umls-concept:C0043393 | lld:lifeskim |
| pubmed-article:1863470 | lifeskim:mentions | umls-concept:C1704689 | lld:lifeskim |
| pubmed-article:1863470 | lifeskim:mentions | umls-concept:C1522642 | lld:lifeskim |
| pubmed-article:1863470 | lifeskim:mentions | umls-concept:C0006556 | lld:lifeskim |
| pubmed-article:1863470 | lifeskim:mentions | umls-concept:C0079600 | lld:lifeskim |
| pubmed-article:1863470 | lifeskim:mentions | umls-concept:C1708096 | lld:lifeskim |
| pubmed-article:1863470 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:1863470 | lifeskim:mentions | umls-concept:C1415205 | lld:lifeskim |
| pubmed-article:1863470 | lifeskim:mentions | umls-concept:C0679058 | lld:lifeskim |
| pubmed-article:1863470 | lifeskim:mentions | umls-concept:C0205164 | lld:lifeskim |
| pubmed-article:1863470 | lifeskim:mentions | umls-concept:C1547699 | lld:lifeskim |
| pubmed-article:1863470 | lifeskim:mentions | umls-concept:C2700640 | lld:lifeskim |
| pubmed-article:1863470 | lifeskim:mentions | umls-concept:C2911684 | lld:lifeskim |
| pubmed-article:1863470 | lifeskim:mentions | umls-concept:C0913822 | lld:lifeskim |
| pubmed-article:1863470 | lifeskim:mentions | umls-concept:C0913823 | lld:lifeskim |
| pubmed-article:1863470 | lifeskim:mentions | umls-concept:C0185117 | lld:lifeskim |
| pubmed-article:1863470 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:1863470 | pubmed:dateCreated | 1991-9-6 | lld:pubmed |
| pubmed-article:1863470 | pubmed:abstractText | A cDNA clone corresponding to the gp41 gene fragment nucl. 7573-7730 of the human immunodeficiency virus type 1 (HIV-1) was selected from a random HIV-1 genomic library expressed in yeast. This clone encodes a 52-residue long peptide (amino acid (a.a.)) 591-642) bearing the major immunodominant domain (a.a. 598-609) of the HIV-1 transmembrane glycoprotein gp41. Expression of the recombinant peptide pSE-env591-642 was driven by the alpha-mating factor leader sequence contained in a plasmid pSE-x allowing the synthesis and secretion of foreign gene product in Saccharomyces cerevisiae. Time-course analysis of the secretion into culture medium revealed an optimal production of the glycoprotein fragment at 28-30 h with no observable cytotoxicity. The secreted peptide is highly glycosylated with NH2-terminal heterogeneity probably due to different post-translational modifications. The secreted peptide shows an extreme antigenicity since in ELISA assays, as few as 5 microliters/well of crude supernatant are sufficient to obtain a strong detection by monoclonal antibodies or by 100% of sera from HIV-infected individuals. The purified glycopeptide pSE-env591-642 binds to a monoclonal antibody directed against the immunodominant epitope (a.a. 603-609) with an affinity similar to that of the complete glycoprotein gp160 (Kd values within the 10(-10) M range) and with a 100-fold higher affinity than that of a linear peptide fragment SP-env584-609. These results indicate that overexpression in yeast can efficiently provide an abundant source of highly antigenic gp41 protein fragment pSE-env591-642 which retains the antigenic properties of the native gp160 protein. Such a recombinant peptide should therefore be considered as a good candidate for antigen in HIV detection tests. | lld:pubmed |
| pubmed-article:1863470 | pubmed:language | eng | lld:pubmed |
| pubmed-article:1863470 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1863470 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:1863470 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1863470 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1863470 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1863470 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1863470 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1863470 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1863470 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1863470 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1863470 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:1863470 | pubmed:month | Apr | lld:pubmed |
| pubmed-article:1863470 | pubmed:issn | 0920-8534 | lld:pubmed |
| pubmed-article:1863470 | pubmed:author | pubmed-author:RossierJJ | lld:pubmed |
| pubmed-article:1863470 | pubmed:author | pubmed-author:GairinJ EJE | lld:pubmed |
| pubmed-article:1863470 | pubmed:author | pubmed-author:TraincardFF | lld:pubmed |
| pubmed-article:1863470 | pubmed:author | pubmed-author:MadaulePP | lld:pubmed |
| pubmed-article:1863470 | pubmed:author | pubmed-author:BarrèsEE | lld:pubmed |
| pubmed-article:1863470 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:1863470 | pubmed:volume | 3 | lld:pubmed |
| pubmed-article:1863470 | pubmed:geneSymbol | pol | lld:pubmed |
| pubmed-article:1863470 | pubmed:geneSymbol | env | lld:pubmed |
| pubmed-article:1863470 | pubmed:geneSymbol | gag | lld:pubmed |
| pubmed-article:1863470 | pubmed:geneSymbol | MF&agr;-1 | lld:pubmed |
| pubmed-article:1863470 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:1863470 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:1863470 | pubmed:pagination | 109-19 | lld:pubmed |
| pubmed-article:1863470 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
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| pubmed-article:1863470 | pubmed:meshHeading | pubmed-meshheading:1863470-... | lld:pubmed |
| pubmed-article:1863470 | pubmed:year | 1991 | lld:pubmed |
| pubmed-article:1863470 | pubmed:articleTitle | Expression in yeast of a cDNA clone encoding a transmembrane glycoprotein gp41 fragment (a.a. 591-642) bearing the major immunodominant domain of human immunodeficiency virus. | lld:pubmed |
| pubmed-article:1863470 | pubmed:affiliation | Laboratoire de Physiologie Nerveuse, Centre National de la Recherche Scientifique, Gif-sur-Yvette, France. | lld:pubmed |
| pubmed-article:1863470 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:1863470 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1863470 | lld:pubmed |
