| pubmed-article:18620910 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:18620910 | lifeskim:mentions | umls-concept:C0025663 | lld:lifeskim |
| pubmed-article:18620910 | lifeskim:mentions | umls-concept:C0683150 | lld:lifeskim |
| pubmed-article:18620910 | lifeskim:mentions | umls-concept:C0008562 | lld:lifeskim |
| pubmed-article:18620910 | lifeskim:mentions | umls-concept:C0037813 | lld:lifeskim |
| pubmed-article:18620910 | lifeskim:mentions | umls-concept:C0205352 | lld:lifeskim |
| pubmed-article:18620910 | lifeskim:mentions | umls-concept:C1142863 | lld:lifeskim |
| pubmed-article:18620910 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:18620910 | pubmed:dateCreated | 2008-8-4 | lld:pubmed |
| pubmed-article:18620910 | pubmed:abstractText | We have developed a simple and reliable method for determining plasma concentration of dehydroxymethylepoxyquinomicin (DHMEQ), a new low molecular weight NF-kappaB inhibitor, using high performance liquid chromatography with mass spectrometry (LC-MS). An experiment of mass spectrometry with electrospray ionization in the negative ionization mode was performed to detect ion transitions at m/z 260.05 [M-H](-) for DHMEQ and 240.29 for mefenamic acid as an internal standard. The samples were purified using liquid-liquid extraction with ethyl acetate. The method yielded a standard curve which was linear for the concentration range of 0.1-125 ng/mL when 0.05 mL plasma was used. The correlation coefficients of all standard curves were greater than or equal to 0.999. The limit of detection was 50 pg/mL (signal/noise >3). Daily fluctuation of plasma standard curve was small. The intra- and inter-assay precision ranged from 2.84 to 4.76% (n=6) and 2.91 to 7.03% (n=6), respectively. The LC-MS technique described provides a simple and reliable liquid chromatographic method for the determination of DHMEQ level and for use in studies involving pharmacokinetics. | lld:pubmed |
| pubmed-article:18620910 | pubmed:language | eng | lld:pubmed |
| pubmed-article:18620910 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18620910 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:18620910 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18620910 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18620910 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18620910 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:18620910 | pubmed:month | Aug | lld:pubmed |
| pubmed-article:18620910 | pubmed:issn | 1570-0232 | lld:pubmed |
| pubmed-article:18620910 | pubmed:author | pubmed-author:WatanabeEtsuk... | lld:pubmed |
| pubmed-article:18620910 | pubmed:author | pubmed-author:OhnoKeikoK | lld:pubmed |
| pubmed-article:18620910 | pubmed:author | pubmed-author:TodoSatoruS | lld:pubmed |
| pubmed-article:18620910 | pubmed:author | pubmed-author:UmezawaKazuoK | lld:pubmed |
| pubmed-article:18620910 | pubmed:author | pubmed-author:FurukawaHiroy... | lld:pubmed |
| pubmed-article:18620910 | pubmed:author | pubmed-author:OzakiMichitak... | lld:pubmed |
| pubmed-article:18620910 | pubmed:author | pubmed-author:KishinoSatosh... | lld:pubmed |
| pubmed-article:18620910 | pubmed:author | pubmed-author:MochizukiNobu... | lld:pubmed |
| pubmed-article:18620910 | pubmed:author | pubmed-author:FukaiMotoM | lld:pubmed |
| pubmed-article:18620910 | pubmed:author | pubmed-author:ShiinoMitsuhi... | lld:pubmed |
| pubmed-article:18620910 | pubmed:author | pubmed-author:AjimaHidetomo... | lld:pubmed |
| pubmed-article:18620910 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:18620910 | pubmed:day | 1 | lld:pubmed |
| pubmed-article:18620910 | pubmed:volume | 871 | lld:pubmed |
| pubmed-article:18620910 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:18620910 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:18620910 | pubmed:pagination | 32-6 | lld:pubmed |
| pubmed-article:18620910 | pubmed:meshHeading | pubmed-meshheading:18620910... | lld:pubmed |
| pubmed-article:18620910 | pubmed:meshHeading | pubmed-meshheading:18620910... | lld:pubmed |
| pubmed-article:18620910 | pubmed:meshHeading | pubmed-meshheading:18620910... | lld:pubmed |
| pubmed-article:18620910 | pubmed:meshHeading | pubmed-meshheading:18620910... | lld:pubmed |
| pubmed-article:18620910 | pubmed:meshHeading | pubmed-meshheading:18620910... | lld:pubmed |
| pubmed-article:18620910 | pubmed:meshHeading | pubmed-meshheading:18620910... | lld:pubmed |
| pubmed-article:18620910 | pubmed:meshHeading | pubmed-meshheading:18620910... | lld:pubmed |
| pubmed-article:18620910 | pubmed:meshHeading | pubmed-meshheading:18620910... | lld:pubmed |
| pubmed-article:18620910 | pubmed:meshHeading | pubmed-meshheading:18620910... | lld:pubmed |
| pubmed-article:18620910 | pubmed:meshHeading | pubmed-meshheading:18620910... | lld:pubmed |
| pubmed-article:18620910 | pubmed:meshHeading | pubmed-meshheading:18620910... | lld:pubmed |
| pubmed-article:18620910 | pubmed:meshHeading | pubmed-meshheading:18620910... | lld:pubmed |
| pubmed-article:18620910 | pubmed:year | 2008 | lld:pubmed |
| pubmed-article:18620910 | pubmed:articleTitle | A simple and reliable method for determining plasma concentration of dehydroxymethylepoxyquinomicin by high performance liquid chromatography with mass spectrometry. | lld:pubmed |
| pubmed-article:18620910 | pubmed:affiliation | Department of Medication Use Analysis and Clinical Research, Meiji Pharmaceutical University, Kiyose, Tokyo, Japan. | lld:pubmed |
| pubmed-article:18620910 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:18620910 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
