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pubmed-article:18576004rdf:typepubmed:Citationlld:pubmed
pubmed-article:18576004lifeskim:mentionsumls-concept:C0010453lld:lifeskim
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pubmed-article:18576004lifeskim:mentionsumls-concept:C0549178lld:lifeskim
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pubmed-article:18576004pubmed:dateCreated2008-6-25lld:pubmed
pubmed-article:18576004pubmed:abstractTextThe continuous cofermentation performance of xylose-fermenting Zymomonas mobilis at 30 degrees C and pH 5.5 was characterized using a pure-sugar feed solution that contained 8 g/L glucose and 40 g/L xylose. Successful chemostat start up resulted in complete utilization of glucose and greater than 85% utilization of xylose, but was only reproducibly achieved using initial dilution rates at or less than 0.04/h; once initiated, cofermentation could be maintained at dilution rates of 0.04 to 0.10/h. Whereas xylose and cell-mass concentrations increased gradually with increasing dilution rate, ethanol concentrations and ethanol yields on available sugars remained approximately constant at 20-22 g/L and 80-90% of theoretical, respectively. Volumetric and specific ethanol productivities increased linearly with increasing dilution rate, rising from approx 1.0 each (g/L/h or g/g/h) at a dilution rate of 0.04/h to approx 2.0 each (g/L/h or g/g/h) at a dilution rate of 0.10/h. Similarly, specific sugar-utilization rates increased from approx 2.0 g/g/h at dilution rate 0.04/h to approx 3.5 g/g/h at dilution rate of 0.10/h. The estimated values of 0.042 g/g for the maximum Z. mobilis cell-mass yield on substrate and 1.13 g/g/h for the minimum specific substrate utilization rate required for cellular maintenance energy are within the range of values reported in the literature. Results are also presented which suggest that long-term adaptation in continuous culture is a powerful technique for developing strains with higher tolerance to inhibitory hemicellulose hydrolyzates.lld:pubmed
pubmed-article:18576004pubmed:languageenglld:pubmed
pubmed-article:18576004pubmed:journalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:18576004pubmed:statusPubMed-not-MEDLINElld:pubmed
pubmed-article:18576004pubmed:issn0273-2289lld:pubmed
pubmed-article:18576004pubmed:authorpubmed-author:LawfordH GHGlld:pubmed
pubmed-article:18576004pubmed:authorpubmed-author:HauckKKlld:pubmed
pubmed-article:18576004pubmed:authorpubmed-author:McMillanJ DJDlld:pubmed
pubmed-article:18576004pubmed:authorpubmed-author:MohagheghiAAlld:pubmed
pubmed-article:18576004pubmed:issnTypePrintlld:pubmed
pubmed-article:18576004pubmed:volume70-72lld:pubmed
pubmed-article:18576004pubmed:ownerNLMlld:pubmed
pubmed-article:18576004pubmed:authorsCompleteYlld:pubmed
pubmed-article:18576004pubmed:pagination353-67lld:pubmed
pubmed-article:18576004pubmed:year1998lld:pubmed
pubmed-article:18576004pubmed:articleTitleContinuous culture studies of xylose-fermenting Zymomonas mobilis.lld:pubmed
pubmed-article:18576004pubmed:affiliationBioengineering Laboratory, Department of Biochemistry, University of Toronto, Toronto, Ontario, Canada M5S 1A8.lld:pubmed
pubmed-article:18576004pubmed:publicationTypeJournal Articlelld:pubmed