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pubmed-article:18547122pubmed:abstractTextEncapsulation of cells and tissue offers a rational approach for attenuating deleterious host responses toward transplanted cells, but a need exists to develop cell encapsulation strategies that minimize transplant volume. In this report, we describe the formation of nanothin, PEG-rich conformal coatings on individual pancreatic islets via layer-by-layer self-assembly of poly( l-lysine)- g-poly(ethylene glycol)(biotin) (PPB) and streptavidin (SA). Through control of grafting ratio, PPB could be rendered nontoxic and facilitated growth of PPB/SA multilayer thin films that conformed to the heterogeneous islet surface. (PPB/SA) 8 multilayer films could be assembled without loss of islet viability or function, and coated islets performed comparably to untreated controls in vivo in a murine model of allogenic intraportal islet transplantation.lld:pubmed
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pubmed-article:18547122pubmed:articleTitleLayer-by-layer assembly of a conformal nanothin PEG coating for intraportal islet transplantation.lld:pubmed
pubmed-article:18547122pubmed:affiliationDepartment of Biomedical Engineering, Georgia Institute of Technology, Atlanta, Georgia 30332, USA.lld:pubmed
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pubmed-article:18547122pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed
pubmed-article:18547122pubmed:publicationTypeResearch Support, N.I.H., Extramurallld:pubmed
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