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pubmed-article:1840924pubmed:abstractTextPlants carrying floury-2, Defective endosperm-B30, or Mucronate mutations overproduce b-70, a maize homolog of the mammalian immunoglobulin binding protein. During endosperm development in these mutants, levels of both b-70 protein and RNA increase dramatically between 14 days and 20 days after pollination. At later stages, b-70 RNA levels decline while protein levels remain high. The increase in b-70 RNA levels is endosperm specific and dependent on gene dosage in the floury-2 mutant. In all three mutants, the increases in b-70 RNA and protein levels are inversely proportional to changes in zein synthesis. Although b-70 polypeptides can be extracted from purified protein bodies, they carry a carboxy-terminal endoplasmic reticulum retention signal, HDEL. We propose that induction of b-70 in these mutants is a cellular response to abnormally folded or improperly assembled storage proteins and probably reflects its role as a polypeptide chain binding protein.lld:pubmed
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pubmed-article:1840924pubmed:articleTitleIncreased expression of the maize immunoglobulin binding protein homolog b-70 in three zein regulatory mutants.lld:pubmed
pubmed-article:1840924pubmed:affiliationDepartment of Botany, North Carolina State University, Raleigh 27695-7612.lld:pubmed
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pubmed-article:1840924pubmed:publicationTypeResearch Support, U.S. Gov't, P.H.S.lld:pubmed
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