| pubmed-article:18262585 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:18262585 | lifeskim:mentions | umls-concept:C0033684 | lld:lifeskim |
| pubmed-article:18262585 | lifeskim:mentions | umls-concept:C1260956 | lld:lifeskim |
| pubmed-article:18262585 | lifeskim:mentions | umls-concept:C0220781 | lld:lifeskim |
| pubmed-article:18262585 | lifeskim:mentions | umls-concept:C1883254 | lld:lifeskim |
| pubmed-article:18262585 | lifeskim:mentions | umls-concept:C0303920 | lld:lifeskim |
| pubmed-article:18262585 | lifeskim:mentions | umls-concept:C0243071 | lld:lifeskim |
| pubmed-article:18262585 | lifeskim:mentions | umls-concept:C0871161 | lld:lifeskim |
| pubmed-article:18262585 | lifeskim:mentions | umls-concept:C0596335 | lld:lifeskim |
| pubmed-article:18262585 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:18262585 | pubmed:dateCreated | 2008-3-10 | lld:pubmed |
| pubmed-article:18262585 | pubmed:abstractText | Green fluorescent protein (GFP) and homologous proteins possess a unique pathway of chromophore formation based on autocatalytic modification of their own amino acid residues. Green-to-red photoconvertible fluorescent protein Kaede carries His-Tyr-Gly chromophore-forming triad. Here, we describe synthesis of Kaede red chromophore (2-[(1E)-2-(5-imidazolyl)ethenyl]-4-(p-hydroxybenzylidene)-5-imidazolone) and its analogs that can be potentially formed by natural amino acid residues. Chromophores corresponding to the following tripeptides were obtained: His-Tyr-Gly, Trp-Tyr-Gly, Phe-Trp-Gly, Tyr-Trp-Gly, Asn-Tyr-Gly, Phe-Tyr-Gly, and Tyr-Tyr-Gly. In basic conditions they fluoresced red with relatively high quantum yield (up to 0.017 for Trp-derived compounds). The most red-shifted absorption peak at 595nm was found for the chromophore Trp-Tyr-Gly in basic DMSO. Surprisingly, in basic DMF non-aromatic Asn-derived chromophore Asn-Tyr-Gly demonstrated the most red-shifted emission maximum at 642 nm. Thus, Asn residue may be a promising substituent, which can potentially diversify posttranslational chemistry in GFP-like proteins. | lld:pubmed |
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| pubmed-article:18262585 | pubmed:language | eng | lld:pubmed |
| pubmed-article:18262585 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18262585 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:18262585 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18262585 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18262585 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18262585 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18262585 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18262585 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:18262585 | pubmed:month | Apr | lld:pubmed |
| pubmed-article:18262585 | pubmed:issn | 1090-2120 | lld:pubmed |
| pubmed-article:18262585 | pubmed:author | pubmed-author:LukyanovKonst... | lld:pubmed |
| pubmed-article:18262585 | pubmed:author | pubmed-author:LukyanovSerge... | lld:pubmed |
| pubmed-article:18262585 | pubmed:author | pubmed-author:YampolskyIlia... | lld:pubmed |
| pubmed-article:18262585 | pubmed:author | pubmed-author:PotapovVictor... | lld:pubmed |
| pubmed-article:18262585 | pubmed:author | pubmed-author:ShcherboDmitr... | lld:pubmed |
| pubmed-article:18262585 | pubmed:author | pubmed-author:KislukhinAlex... | lld:pubmed |
| pubmed-article:18262585 | pubmed:author | pubmed-author:AmatovTynchty... | lld:pubmed |
| pubmed-article:18262585 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:18262585 | pubmed:volume | 36 | lld:pubmed |
| pubmed-article:18262585 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:18262585 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:18262585 | pubmed:pagination | 96-104 | lld:pubmed |
| pubmed-article:18262585 | pubmed:dateRevised | 2011-9-26 | lld:pubmed |
| pubmed-article:18262585 | pubmed:meshHeading | pubmed-meshheading:18262585... | lld:pubmed |
| pubmed-article:18262585 | pubmed:meshHeading | pubmed-meshheading:18262585... | lld:pubmed |
| pubmed-article:18262585 | pubmed:meshHeading | pubmed-meshheading:18262585... | lld:pubmed |
| pubmed-article:18262585 | pubmed:meshHeading | pubmed-meshheading:18262585... | lld:pubmed |
| pubmed-article:18262585 | pubmed:meshHeading | pubmed-meshheading:18262585... | lld:pubmed |
| pubmed-article:18262585 | pubmed:meshHeading | pubmed-meshheading:18262585... | lld:pubmed |
| pubmed-article:18262585 | pubmed:year | 2008 | lld:pubmed |
| pubmed-article:18262585 | pubmed:articleTitle | Synthesis and properties of the red chromophore of the green-to-red photoconvertible fluorescent protein Kaede and its analogs. | lld:pubmed |
| pubmed-article:18262585 | pubmed:affiliation | Institute of Bioorganic Chemistry, Lab of Molecular Techonologies, Miklukho-Maklaya 16/10, Moscow 117997, Russian Federation. | lld:pubmed |
| pubmed-article:18262585 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:18262585 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| pubmed-article:18262585 | pubmed:publicationType | Research Support, N.I.H., Extramural | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:18262585 | lld:pubmed |
