| pubmed-article:18077411 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:18077411 | lifeskim:mentions | umls-concept:C0162570 | lld:lifeskim |
| pubmed-article:18077411 | lifeskim:mentions | umls-concept:C0920283 | lld:lifeskim |
| pubmed-article:18077411 | lifeskim:mentions | umls-concept:C0302523 | lld:lifeskim |
| pubmed-article:18077411 | lifeskim:mentions | umls-concept:C0700325 | lld:lifeskim |
| pubmed-article:18077411 | lifeskim:mentions | umls-concept:C0441712 | lld:lifeskim |
| pubmed-article:18077411 | lifeskim:mentions | umls-concept:C0913822 | lld:lifeskim |
| pubmed-article:18077411 | lifeskim:mentions | umls-concept:C0913823 | lld:lifeskim |
| pubmed-article:18077411 | pubmed:issue | 50 | lld:pubmed |
| pubmed-article:18077411 | pubmed:dateCreated | 2007-12-13 | lld:pubmed |
| pubmed-article:18077411 | pubmed:abstractText | Helicases are enzymes that couple ATP hydrolysis to the unwinding of double-stranded (ds) nucleic acids. The bacteriophage T4 helicase (gp41) is a hexameric helicase that promotes DNA replication within a highly coordinated protein complex termed the replisome. Despite recent progress, the gp41 unwinding mechanism and regulatory interactions within the replisome remain unclear. Here we use a single tethered DNA hairpin as a real-time reporter of gp41-mediated dsDNA unwinding and single-stranded (ss) DNA translocation with 3-base pair (bp) resolution. Although gp41 translocates on ssDNA as fast as the in vivo replication fork ( approximately 400 bp/s), its unwinding rate extrapolated to zero force is much slower ( approximately 30 bp/s). Together, our results have two implications: first, gp41 unwinds DNA through a passive mechanism; second, this weak helicase cannot efficiently unwind the T4 genome alone. Our results suggest that important regulations occur within the replisome to achieve rapid and processive replication. | lld:pubmed |
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| pubmed-article:18077411 | pubmed:language | eng | lld:pubmed |
| pubmed-article:18077411 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18077411 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:18077411 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:18077411 | pubmed:month | Dec | lld:pubmed |
| pubmed-article:18077411 | pubmed:issn | 1091-6490 | lld:pubmed |
| pubmed-article:18077411 | pubmed:author | pubmed-author:BenkovicSteph... | lld:pubmed |
| pubmed-article:18077411 | pubmed:author | pubmed-author:CroquetteVinc... | lld:pubmed |
| pubmed-article:18077411 | pubmed:author | pubmed-author:SpieringMiche... | lld:pubmed |
| pubmed-article:18077411 | pubmed:author | pubmed-author:BensimonDavid... | lld:pubmed |
| pubmed-article:18077411 | pubmed:author | pubmed-author:LionnetTimoth... | lld:pubmed |
| pubmed-article:18077411 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:18077411 | pubmed:day | 11 | lld:pubmed |
| pubmed-article:18077411 | pubmed:volume | 104 | lld:pubmed |
| pubmed-article:18077411 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:18077411 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:18077411 | pubmed:pagination | 19790-5 | lld:pubmed |
| pubmed-article:18077411 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
| pubmed-article:18077411 | pubmed:meshHeading | pubmed-meshheading:18077411... | lld:pubmed |
| pubmed-article:18077411 | pubmed:meshHeading | pubmed-meshheading:18077411... | lld:pubmed |
| pubmed-article:18077411 | pubmed:meshHeading | pubmed-meshheading:18077411... | lld:pubmed |
| pubmed-article:18077411 | pubmed:meshHeading | pubmed-meshheading:18077411... | lld:pubmed |
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| pubmed-article:18077411 | pubmed:meshHeading | pubmed-meshheading:18077411... | lld:pubmed |
| pubmed-article:18077411 | pubmed:year | 2007 | lld:pubmed |
| pubmed-article:18077411 | pubmed:articleTitle | Real-time observation of bacteriophage T4 gp41 helicase reveals an unwinding mechanism. | lld:pubmed |
| pubmed-article:18077411 | pubmed:affiliation | Laboratoire de Physique Statistique, Ecole Normale Supérieure, Centre National de la Recherche Scientifique-UMR8550, 24 Rue Lhomond, 75005 Paris, France. tlionnet@aecom.yu.edu | lld:pubmed |
| pubmed-article:18077411 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:18077411 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| pubmed-article:18077411 | pubmed:publicationType | Research Support, N.I.H., Extramural | lld:pubmed |
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