| pubmed-article:18023288 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:18023288 | lifeskim:mentions | umls-concept:C0338106 | lld:lifeskim |
| pubmed-article:18023288 | lifeskim:mentions | umls-concept:C1622501 | lld:lifeskim |
| pubmed-article:18023288 | lifeskim:mentions | umls-concept:C1510779 | lld:lifeskim |
| pubmed-article:18023288 | lifeskim:mentions | umls-concept:C0038836 | lld:lifeskim |
| pubmed-article:18023288 | lifeskim:mentions | umls-concept:C2587213 | lld:lifeskim |
| pubmed-article:18023288 | lifeskim:mentions | umls-concept:C0033268 | lld:lifeskim |
| pubmed-article:18023288 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:18023288 | pubmed:dateCreated | 2008-1-14 | lld:pubmed |
| pubmed-article:18023288 | pubmed:abstractText | Reactive oxygen species are well-known mediators of various biological responses. Recently, new homologues of the catalytic subunit of NADPH oxidase have been discovered in non-phagocytic cells. These new homologues (Nox1-Nox5) produce low levels of superoxides compared to the phagocytic homologue Nox2/gp91phox. Using Nox1 siRNA, we show that Nox1-dependent superoxide production affects the migration of HT29-D4 colonic adenocarcinoma cells on collagen-I. Nox1 inhibition or down-regulation led to a decrease of superoxide production and alpha 2 beta 1 integrin membrane availability. An addition of arachidonic acid stimulated Nox1-dependent superoxide production and HT29-D4 cell migration. Pharmacological evidences using phospholipase A2, lipoxygenases and protein kinase C inhibitors show that upstream regulation of Nox1 relies on arachidonic acid metabolism. Inhibition of 12-lipoxygenase decreased basal and arachidonic acid induced Nox1-dependent superoxide production and cell migration. Migration and ROS production inhibited by a 12-lipoxygenase inhibitor were restored by the addition of 12(S)-HETE, a downstream product of 12-lipoxygenase. Protein kinase C delta inhibition by rottlerin (and also GO6983) prevented Nox1-dependent superoxide production and inhibited cell migration, while other protein kinase C inhibitors were ineffective. We conclude that Nox1 activation by arachidonic acid metabolism occurs through 12-lipoxygenase and protein kinase C delta, and controls cell migration by affecting integrin alpha 2 subunit turn-over. | lld:pubmed |
| pubmed-article:18023288 | pubmed:language | eng | lld:pubmed |
| pubmed-article:18023288 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18023288 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:18023288 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:18023288 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:18023288 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:18023288 | pubmed:month | Jan | lld:pubmed |
| pubmed-article:18023288 | pubmed:issn | 0006-3002 | lld:pubmed |
| pubmed-article:18023288 | pubmed:author | pubmed-author:PenelClaudeC | lld:pubmed |
| pubmed-article:18023288 | pubmed:author | pubmed-author:GattaccecaFlo... | lld:pubmed |
| pubmed-article:18023288 | pubmed:author | pubmed-author:KovacicHervéH | lld:pubmed |
| pubmed-article:18023288 | pubmed:author | pubmed-author:Bourgarel-Rey... | lld:pubmed |
| pubmed-article:18023288 | pubmed:author | pubmed-author:LehmannMaxime... | lld:pubmed |
| pubmed-article:18023288 | pubmed:author | pubmed-author:SadokAmineA | lld:pubmed |
| pubmed-article:18023288 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:18023288 | pubmed:volume | 1783 | lld:pubmed |
| pubmed-article:18023288 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:18023288 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:18023288 | pubmed:pagination | 23-33 | lld:pubmed |
| pubmed-article:18023288 | pubmed:dateRevised | 2009-11-19 | lld:pubmed |
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| pubmed-article:18023288 | pubmed:meshHeading | pubmed-meshheading:18023288... | lld:pubmed |
| pubmed-article:18023288 | pubmed:year | 2008 | lld:pubmed |
| pubmed-article:18023288 | pubmed:articleTitle | Nox1-dependent superoxide production controls colon adenocarcinoma cell migration. | lld:pubmed |
| pubmed-article:18023288 | pubmed:affiliation | CNRS FRE 2737, Cytosquelette et Intégration des Signaux du Microenvironnement Tumoral, Aix-Marseille Université, France. | lld:pubmed |
| pubmed-article:18023288 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:18023288 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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