| pubmed-article:17906137 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:17906137 | lifeskim:mentions | umls-concept:C0010156 | lld:lifeskim |
| pubmed-article:17906137 | lifeskim:mentions | umls-concept:C0033684 | lld:lifeskim |
| pubmed-article:17906137 | lifeskim:mentions | umls-concept:C0205245 | lld:lifeskim |
| pubmed-article:17906137 | lifeskim:mentions | umls-concept:C0678594 | lld:lifeskim |
| pubmed-article:17906137 | lifeskim:mentions | umls-concept:C0871161 | lld:lifeskim |
| pubmed-article:17906137 | pubmed:issue | Pt 10 | lld:pubmed |
| pubmed-article:17906137 | pubmed:dateCreated | 2007-10-1 | lld:pubmed |
| pubmed-article:17906137 | pubmed:abstractText | Arcanobacterium pyogenes, an opportunistic pathogen of economically important food animals, is the causative agent of liver abscesses in feedlot cattle, osteomyelitis in turkeys, and pneumonia and arthritis in pigs. Previous studies identified the first A. pyogenes adhesin, CbpA, a protein located on the bacterial surface which has the ability to bind collagen and promotes adhesion to the host cells. The protein has an N-terminal ligand-binding region (region A) and a C-terminal repetitive domain (region B). In this study we found that CbpA bound to almost all the collagen types tested but not to other proteins, and it displayed a propensity to interact with several collagenous peptides derived by CNBr cleavage of type I and II collagens. The K(D) values of CbpA for type I and II collagens and collagen peptides determined by solid-phase binding assay and intrinsic tryptophan fluorescence were in the range of 1-15 nM. It was also found that CbpA and its A region bound fibronectin, and that collagen and fibronectin interacted with distinct subsites. Anti-CbpA antibodies were effective at inhibiting both binding of isolated CbpA and bacterial adhesion to immobilized collagen, suggesting that CbpA is a functional collagen-binding adhesin. Analysis of the immunological cross-reactivity of CbpA with antibodies against other bacterial collagen-binding proteins indicated that CbpA is immunologically related to ACE from Enterococcus faecalis but not to CNA from Staphylococcus aureus or Acm from Enterococcus faecium. Far-UV and near-UV circular dichroism spectra showed that full-length CbpA and its region A are mainly composed of beta-sheet with only a minor alpha-helical component and that both the proteins have a well-defined tertiary structure. | lld:pubmed |
| pubmed-article:17906137 | pubmed:language | eng | lld:pubmed |
| pubmed-article:17906137 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17906137 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:17906137 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17906137 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17906137 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17906137 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17906137 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17906137 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17906137 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17906137 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17906137 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17906137 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17906137 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:17906137 | pubmed:month | Oct | lld:pubmed |
| pubmed-article:17906137 | pubmed:issn | 1350-0872 | lld:pubmed |
| pubmed-article:17906137 | pubmed:author | pubmed-author:JostB HelenBH | lld:pubmed |
| pubmed-article:17906137 | pubmed:author | pubmed-author:RindiSimonett... | lld:pubmed |
| pubmed-article:17906137 | pubmed:author | pubmed-author:SpezialePietr... | lld:pubmed |
| pubmed-article:17906137 | pubmed:author | pubmed-author:PietrocolaGia... | lld:pubmed |
| pubmed-article:17906137 | pubmed:author | pubmed-author:ValtulinaVivi... | lld:pubmed |
| pubmed-article:17906137 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:17906137 | pubmed:volume | 153 | lld:pubmed |
| pubmed-article:17906137 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:17906137 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:17906137 | pubmed:pagination | 3380-9 | lld:pubmed |
| pubmed-article:17906137 | pubmed:meshHeading | pubmed-meshheading:17906137... | lld:pubmed |
| pubmed-article:17906137 | pubmed:meshHeading | pubmed-meshheading:17906137... | lld:pubmed |
| pubmed-article:17906137 | pubmed:meshHeading | pubmed-meshheading:17906137... | lld:pubmed |
| pubmed-article:17906137 | pubmed:meshHeading | pubmed-meshheading:17906137... | lld:pubmed |
| pubmed-article:17906137 | pubmed:meshHeading | pubmed-meshheading:17906137... | lld:pubmed |
| pubmed-article:17906137 | pubmed:meshHeading | pubmed-meshheading:17906137... | lld:pubmed |
| pubmed-article:17906137 | pubmed:meshHeading | pubmed-meshheading:17906137... | lld:pubmed |
| pubmed-article:17906137 | pubmed:meshHeading | pubmed-meshheading:17906137... | lld:pubmed |
| pubmed-article:17906137 | pubmed:meshHeading | pubmed-meshheading:17906137... | lld:pubmed |
| pubmed-article:17906137 | pubmed:meshHeading | pubmed-meshheading:17906137... | lld:pubmed |
| pubmed-article:17906137 | pubmed:meshHeading | pubmed-meshheading:17906137... | lld:pubmed |
| pubmed-article:17906137 | pubmed:meshHeading | pubmed-meshheading:17906137... | lld:pubmed |
| pubmed-article:17906137 | pubmed:year | 2007 | lld:pubmed |
| pubmed-article:17906137 | pubmed:articleTitle | Functional and structural properties of CbpA, a collagen-binding protein from Arcanobacterium pyogenes. | lld:pubmed |
| pubmed-article:17906137 | pubmed:affiliation | University of Pavia, Department of Biochemistry, Viale Taramelli 3/B, 27100 Pavia, Italy. | lld:pubmed |
| pubmed-article:17906137 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:17906137 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | entrezgene:pubmed | pubmed-article:17906137 | lld:entrezgene |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:17906137 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:17906137 | lld:pubmed |
