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pubmed-article:1783586pubmed:abstractTextA sensitive screening method has been developed for detecting sulfamethazine (SMZ) contamination of feeds by using either polyclonal or monoclonal antibodies and a direct competitive enzyme-linked immunosorbent screening assay (ELISA). Feed samples of 25.0 g are extracted with 0.5N HCl and centrifuged. The extract is adjusted to pH 7.0 with 3.0N NaOH and recentrifuged. This pH-adjusted extract is used in the ELISA. Levels as low as 0.004 micrograms SMZ/g feed were detected in supplemented extracts by polyclonal antibodies; levels of 0.4 micrograms SMZ/g feed were detected by a monoclonal antibody.lld:pubmed
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pubmed-article:1783586pubmed:authorpubmed-author:KatzS ESElld:pubmed
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pubmed-article:1783586pubmed:dateRevised2006-11-15lld:pubmed
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pubmed-article:1783586pubmed:articleTitleCompetitive direct enzyme-linked immunosorbent screening assay for the detection of sulfamethazine contamination of animal feeds.lld:pubmed
pubmed-article:1783586pubmed:affiliationRutgers University, Cook College, Department of Biochemistry and Microbiology, New Brunswick, NJ 08903-0231.lld:pubmed
pubmed-article:1783586pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:1783586pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed