| pubmed-article:17662228 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:17662228 | lifeskim:mentions | umls-concept:C0020792 | lld:lifeskim |
| pubmed-article:17662228 | lifeskim:mentions | umls-concept:C0039142 | lld:lifeskim |
| pubmed-article:17662228 | lifeskim:mentions | umls-concept:C0033684 | lld:lifeskim |
| pubmed-article:17662228 | lifeskim:mentions | umls-concept:C2349943 | lld:lifeskim |
| pubmed-article:17662228 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:17662228 | pubmed:dateCreated | 2007-8-13 | lld:pubmed |
| pubmed-article:17662228 | pubmed:abstractText | In typical mass spectrometry-based protein identification using peptide fragmentation fingerprinting, front-end separation plays a critical role in successful peptide sequencing. This separation step demands a great deal of time and usually is the rate-limiting step for the whole process. Here we provide an alternative separation method, based on a simple nanoflow delivery system, that is able to shorten the separation time considerably. This system consists of a 25-mul syringe connected to a manually packed reversed-phase mini-capillary column that can be directly coupled to an electrospray ionization tandem mass spectrometer. A syringe pump is then used to deliver the peptide mixtures at a nanoscale flow rate. We examined the efficiency and efficacy of this method by analyzing the tryptic peptides of bovine serum albumin and of 10 Escherichia coli proteins separated by two-dimensional gel electrophoresis (2DE). The results showed that identification of each protein could be achieved successfully within 25 min by using the disposable mini-capillary column. Moreover, all 2DE-separated E. coli proteins were identified at high confidence levels. Together, our data suggest that this method is a suitable option for mass spectrometry-based protein identification. | lld:pubmed |
| pubmed-article:17662228 | pubmed:language | eng | lld:pubmed |
| pubmed-article:17662228 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17662228 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:17662228 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17662228 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17662228 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17662228 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17662228 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17662228 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17662228 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:17662228 | pubmed:month | Sep | lld:pubmed |
| pubmed-article:17662228 | pubmed:issn | 0003-2697 | lld:pubmed |
| pubmed-article:17662228 | pubmed:author | pubmed-author:LuChi-YuCY | lld:pubmed |
| pubmed-article:17662228 | pubmed:author | pubmed-author:LinChao-Hsiun... | lld:pubmed |
| pubmed-article:17662228 | pubmed:author | pubmed-author:WuChiu-YiCY | lld:pubmed |
| pubmed-article:17662228 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:17662228 | pubmed:day | 15 | lld:pubmed |
| pubmed-article:17662228 | pubmed:volume | 368 | lld:pubmed |
| pubmed-article:17662228 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:17662228 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:17662228 | pubmed:pagination | 123-9 | lld:pubmed |
| pubmed-article:17662228 | pubmed:meshHeading | pubmed-meshheading:17662228... | lld:pubmed |
| pubmed-article:17662228 | pubmed:meshHeading | pubmed-meshheading:17662228... | lld:pubmed |
| pubmed-article:17662228 | pubmed:meshHeading | pubmed-meshheading:17662228... | lld:pubmed |
| pubmed-article:17662228 | pubmed:meshHeading | pubmed-meshheading:17662228... | lld:pubmed |
| pubmed-article:17662228 | pubmed:meshHeading | pubmed-meshheading:17662228... | lld:pubmed |
| pubmed-article:17662228 | pubmed:meshHeading | pubmed-meshheading:17662228... | lld:pubmed |
| pubmed-article:17662228 | pubmed:meshHeading | pubmed-meshheading:17662228... | lld:pubmed |
| pubmed-article:17662228 | pubmed:meshHeading | pubmed-meshheading:17662228... | lld:pubmed |
| pubmed-article:17662228 | pubmed:meshHeading | pubmed-meshheading:17662228... | lld:pubmed |
| pubmed-article:17662228 | pubmed:meshHeading | pubmed-meshheading:17662228... | lld:pubmed |
| pubmed-article:17662228 | pubmed:meshHeading | pubmed-meshheading:17662228... | lld:pubmed |
| pubmed-article:17662228 | pubmed:meshHeading | pubmed-meshheading:17662228... | lld:pubmed |
| pubmed-article:17662228 | pubmed:meshHeading | pubmed-meshheading:17662228... | lld:pubmed |
| pubmed-article:17662228 | pubmed:meshHeading | pubmed-meshheading:17662228... | lld:pubmed |
| pubmed-article:17662228 | pubmed:year | 2007 | lld:pubmed |
| pubmed-article:17662228 | pubmed:articleTitle | Protein identification by syringe pump-driven reversed-phase LC-MS/MS. | lld:pubmed |
| pubmed-article:17662228 | pubmed:affiliation | Proteome Research Center, National Yang-Ming University, Taipei 11221, Taiwan. | lld:pubmed |
| pubmed-article:17662228 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:17662228 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| pubmed-article:17662228 | pubmed:publicationType | Evaluation Studies | lld:pubmed |
