| pubmed-article:17623778 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:17623778 | lifeskim:mentions | umls-concept:C1882071 | lld:lifeskim |
| pubmed-article:17623778 | lifeskim:mentions | umls-concept:C0033684 | lld:lifeskim |
| pubmed-article:17623778 | lifeskim:mentions | umls-concept:C0249742 | lld:lifeskim |
| pubmed-article:17623778 | lifeskim:mentions | umls-concept:C1412511 | lld:lifeskim |
| pubmed-article:17623778 | lifeskim:mentions | umls-concept:C1418979 | lld:lifeskim |
| pubmed-article:17623778 | lifeskim:mentions | umls-concept:C0392747 | lld:lifeskim |
| pubmed-article:17623778 | lifeskim:mentions | umls-concept:C0162657 | lld:lifeskim |
| pubmed-article:17623778 | pubmed:issue | Pt 15 | lld:pubmed |
| pubmed-article:17623778 | pubmed:dateCreated | 2007-7-24 | lld:pubmed |
| pubmed-article:17623778 | pubmed:abstractText | G-protein-coupled receptors (GPCRs) transduce the binding of extracellular stimuli into intracellular signalling cascades that can lead to morphological changes. Here, we demonstrate that stimulation of the calcium-sensing receptor (CaSR), a GPCR that promotes chemotaxis by detecting increases in extracellular calcium, triggers plasma membrane (PM) ruffling via a pathway that involves beta-arrestin 1, Arf nucleotide binding site opener (ARNO), ADP-ribosylating factor 6 (ARF6) and engulfment and cell motility protein (ELMO). Expression of dominant negative beta-arrestin 1 or its knockdown with siRNA impaired the CaSR-induced PM ruffling response. Expression of a catalytically inactive ARNO also reduced CaSR-induced PM ruffling. Furthermore, beta-arrestin 1 co-immunoprecipitated with the CaSR and ARNO under resting conditions. Agonist treatment did not markedly alter beta-arrestin 1 binding to the CaSR or to ARNO but it did elicit the translocation and colocalisation of the CaSR, beta-arrestin 1 and ARNO to membrane protrusions. Furthermore, ARF6 and ELMO, two proteins known to couple ARNO to the cytoskeleton, were required for CaSR-dependent morphological changes and translocated to the PM ruffles. These data suggest that cells ruffle upon CaSR stimulation via a mechanism that involves translocation of beta-arrestin 1 pre-assembled with the CaSR or ARNO, and that ELMO plays an essential role in this CaSR-signalling-induced cytoskeletal reorganisation. | lld:pubmed |
| pubmed-article:17623778 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17623778 | pubmed:language | eng | lld:pubmed |
| pubmed-article:17623778 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17623778 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:17623778 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17623778 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17623778 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17623778 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17623778 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17623778 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17623778 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17623778 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17623778 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17623778 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17623778 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17623778 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:17623778 | pubmed:month | Aug | lld:pubmed |
| pubmed-article:17623778 | pubmed:issn | 0021-9533 | lld:pubmed |
| pubmed-article:17623778 | pubmed:author | pubmed-author:MartinStéphan... | lld:pubmed |
| pubmed-article:17623778 | pubmed:author | pubmed-author:HenleyJeremy... | lld:pubmed |
| pubmed-article:17623778 | pubmed:author | pubmed-author:BouschetTrist... | lld:pubmed |
| pubmed-article:17623778 | pubmed:author | pubmed-author:Venkateswarlu... | lld:pubmed |
| pubmed-article:17623778 | pubmed:author | pubmed-author:MundellStuart... | lld:pubmed |
| pubmed-article:17623778 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:17623778 | pubmed:day | 1 | lld:pubmed |
| pubmed-article:17623778 | pubmed:volume | 120 | lld:pubmed |
| pubmed-article:17623778 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:17623778 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:17623778 | pubmed:pagination | 2489-97 | lld:pubmed |
| pubmed-article:17623778 | pubmed:dateRevised | 2010-10-11 | lld:pubmed |
| pubmed-article:17623778 | pubmed:meshHeading | pubmed-meshheading:17623778... | lld:pubmed |
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| pubmed-article:17623778 | pubmed:meshHeading | pubmed-meshheading:17623778... | lld:pubmed |
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| pubmed-article:17623778 | pubmed:meshHeading | pubmed-meshheading:17623778... | lld:pubmed |
| pubmed-article:17623778 | pubmed:meshHeading | pubmed-meshheading:17623778... | lld:pubmed |
| pubmed-article:17623778 | pubmed:meshHeading | pubmed-meshheading:17623778... | lld:pubmed |
| pubmed-article:17623778 | pubmed:meshHeading | pubmed-meshheading:17623778... | lld:pubmed |
| pubmed-article:17623778 | pubmed:meshHeading | pubmed-meshheading:17623778... | lld:pubmed |
| pubmed-article:17623778 | pubmed:meshHeading | pubmed-meshheading:17623778... | lld:pubmed |
| pubmed-article:17623778 | pubmed:meshHeading | pubmed-meshheading:17623778... | lld:pubmed |
| pubmed-article:17623778 | pubmed:year | 2007 | lld:pubmed |
| pubmed-article:17623778 | pubmed:articleTitle | The calcium-sensing receptor changes cell shape via a beta-arrestin-1 ARNO ARF6 ELMO protein network. | lld:pubmed |
| pubmed-article:17623778 | pubmed:affiliation | Department of Anatomy, MRC Centre for Synaptic Plasticity, University of Bristol, University Walk, Bristol, BS8 1TD, UK. | lld:pubmed |
| pubmed-article:17623778 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:17623778 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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