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pubmed-article:17623723pubmed:abstractTextBlastocyst biogenesis occurs over several cell cycles during the preimplantation period comprising the gradual expression and membrane assembly of junctional protein complexes which distinguish the outer epithelial trophectoderm (TE) cells from the inner cell mass (ICM). In the human, TE integrity and the formation of a junctional seal can often be impaired. Embryos likely to result in a successful pregnancy after transfer are mostly selected according to morphological criteria. Recent data suggest that non-invasive measurement of amino acid turnover may be useful to complement such morphological scores. Whether morphological and metabolic criteria can be linked to poor TE differentiation thereby underpinning developmental predictions mechanistically remains unknown.lld:pubmed
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pubmed-article:17623723pubmed:articleTitleHuman embryos developing in vitro are susceptible to impaired epithelial junction biogenesis correlating with abnormal metabolic activity.lld:pubmed
pubmed-article:17623723pubmed:affiliationSchool of Biological Sciences, University of Southampton, Bassett Crescent East, Southampton, UK. jje@soton.ac.uklld:pubmed
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