| pubmed-article:17562610 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:17562610 | lifeskim:mentions | umls-concept:C0025663 | lld:lifeskim |
| pubmed-article:17562610 | lifeskim:mentions | umls-concept:C0870134 | lld:lifeskim |
| pubmed-article:17562610 | lifeskim:mentions | umls-concept:C0229601 | lld:lifeskim |
| pubmed-article:17562610 | lifeskim:mentions | umls-concept:C1171346 | lld:lifeskim |
| pubmed-article:17562610 | lifeskim:mentions | umls-concept:C0033268 | lld:lifeskim |
| pubmed-article:17562610 | lifeskim:mentions | umls-concept:C0679622 | lld:lifeskim |
| pubmed-article:17562610 | lifeskim:mentions | umls-concept:C0205314 | lld:lifeskim |
| pubmed-article:17562610 | pubmed:issue | 5 | lld:pubmed |
| pubmed-article:17562610 | pubmed:dateCreated | 2007-6-12 | lld:pubmed |
| pubmed-article:17562610 | pubmed:abstractText | We propose a novel method for the efficient production of hematopoietic progenitors from human embryonic stem cells (hESC) via coculture with murine fetal liver-derived stromal cells, in which embryonic hematopoiesis dramatically expands at midgestation. We generated various hematopoietic progenitors in coculture, and this hematopoietic activity was concentrated in cobblestone-like cells derived from differentiated hESC. The cobblestone-like cells mostly expressed CD34 and retained an endothelial cell potential. They also contained hematopoietic colony-forming cells, especially erythroid and multilineage colony-forming cells at high frequency. The multipotential hematopoietic progenitors abundant among the cobblestone-like cells produced almost all types of mature blood cells, including adult-type alpha-globin-expressing erythrocytes and tryptase/chymase double-positive mast cells. These progenitors showed neither the immature properties of ESC nor the potential to differentiate into endoderm and ectoderm at a clonal level. The coculture system developed for hESC can provide a novel source of hematopoietic and blood cells for applications in cellular therapy and drug screening. | lld:pubmed |
| pubmed-article:17562610 | pubmed:language | eng | lld:pubmed |
| pubmed-article:17562610 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17562610 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:17562610 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:17562610 | pubmed:month | Jun | lld:pubmed |
| pubmed-article:17562610 | pubmed:issn | 0925-5710 | lld:pubmed |
| pubmed-article:17562610 | pubmed:author | pubmed-author:DengL JLJ | lld:pubmed |
| pubmed-article:17562610 | pubmed:author | pubmed-author:NakahataTatsu... | lld:pubmed |
| pubmed-article:17562610 | pubmed:author | pubmed-author:WangDanD | lld:pubmed |
| pubmed-article:17562610 | pubmed:author | pubmed-author:KawasakiHiroh... | lld:pubmed |
| pubmed-article:17562610 | pubmed:author | pubmed-author:HeikeToshioT | lld:pubmed |
| pubmed-article:17562610 | pubmed:author | pubmed-author:TsujiKohichir... | lld:pubmed |
| pubmed-article:17562610 | pubmed:author | pubmed-author:EbiharaYasuhi... | lld:pubmed |
| pubmed-article:17562610 | pubmed:author | pubmed-author:HanadaSachiyo... | lld:pubmed |
| pubmed-article:17562610 | pubmed:author | pubmed-author:ZaikeYujiY | lld:pubmed |
| pubmed-article:17562610 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:17562610 | pubmed:volume | 85 | lld:pubmed |
| pubmed-article:17562610 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:17562610 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:17562610 | pubmed:pagination | 371-9 | lld:pubmed |
| pubmed-article:17562610 | pubmed:meshHeading | pubmed-meshheading:17562610... | lld:pubmed |
| pubmed-article:17562610 | pubmed:meshHeading | pubmed-meshheading:17562610... | lld:pubmed |
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| pubmed-article:17562610 | pubmed:meshHeading | pubmed-meshheading:17562610... | lld:pubmed |
| pubmed-article:17562610 | pubmed:meshHeading | pubmed-meshheading:17562610... | lld:pubmed |
| pubmed-article:17562610 | pubmed:meshHeading | pubmed-meshheading:17562610... | lld:pubmed |
| pubmed-article:17562610 | pubmed:meshHeading | pubmed-meshheading:17562610... | lld:pubmed |
| pubmed-article:17562610 | pubmed:meshHeading | pubmed-meshheading:17562610... | lld:pubmed |
| pubmed-article:17562610 | pubmed:meshHeading | pubmed-meshheading:17562610... | lld:pubmed |
| pubmed-article:17562610 | pubmed:meshHeading | pubmed-meshheading:17562610... | lld:pubmed |
| pubmed-article:17562610 | pubmed:meshHeading | pubmed-meshheading:17562610... | lld:pubmed |
| pubmed-article:17562610 | pubmed:meshHeading | pubmed-meshheading:17562610... | lld:pubmed |
| pubmed-article:17562610 | pubmed:meshHeading | pubmed-meshheading:17562610... | lld:pubmed |
| pubmed-article:17562610 | pubmed:meshHeading | pubmed-meshheading:17562610... | lld:pubmed |
| pubmed-article:17562610 | pubmed:year | 2007 | lld:pubmed |
| pubmed-article:17562610 | pubmed:articleTitle | Novel method for efficient production of multipotential hematopoietic progenitors from human embryonic stem cells. | lld:pubmed |
| pubmed-article:17562610 | pubmed:affiliation | Division of Cellular Therapy, Advanced Clinical Research Center, Institute of Medical Science, University of Tokyo, Tokyo, Japan. | lld:pubmed |
| pubmed-article:17562610 | pubmed:publicationType | Journal Article | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:17562610 | lld:pubmed |
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| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:17562610 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:17562610 | lld:pubmed |
