Linked Life Data

17540587

Source:http://linkedlifedata.com/resource/pubmed/id/17540587

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pubmed-article:17540587pubmed:abstractTextThe anaerobic spirochete Brachyspira hyodysenteriae is host to a bacteriophage-like agent known as VSH-1. VSH-1 is a novel gene transfer mechanism which does not self-propagate and transfers random 7.5kb fragments of host DNA between B. hyodysenteriae cells. In these investigations early events during VSH-1 induction by mitomycin C were examined. Quantitative PCR analysis revealed that VSH-1 hvp38 and hvp53 genes did not detectably increase in copy numbers during induction. Based on Northern blot hybridization assays, transcription of VSH-1 genes hvp38, hvp53, hvp45, hvp101, and lys increased fivefold to tenfold between 2 and 4h after induction whereas mRNA levels for B. hyodysenteriae flaA1 declined over the same time period. Chloramphenicol prevented the mitomycin C-induced increases in VSH-1 gene transcription. Hydrogen peroxide (300muM) substituted for mitomycin C as an inducer of VSH-1 gene transcription and is a possible 'natural' inducer of VSH-1 production in vivo. Northern blot hybridization, RT PCR, and primer extension analyses showed that VSH-1 genes are co-transcribed at an initiation site upstream of the VSH-1 gene operon. Two direct heptanucleotide repeats (ACTTATA) were identified between the putative -35 and -10 positions of the VSH-1 gene operon and are likely to represent a binding site for transcription proteins. These findings indicate VSH-1 virion production does not require genome replication, consistent with the inability of VSH-1 to self-propagate. Early events in VSH-1 induction include de novo synthesis of protein(s) essential for transcription of VSH-1 genes as polycistronic mRNA initiating upstream of the hvp45 gene.lld:pubmed
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pubmed-article:17540587pubmed:dateRevised2008-11-21lld:pubmed
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pubmed-article:17540587pubmed:articleTitleInduction and transcription of VSH-1, a prophage-like gene transfer agent of Brachyspira hyodysenteriae.lld:pubmed
pubmed-article:17540587pubmed:affiliationDepartment of Microbiology, Iowa State University, Ames, IA 50010, USA.lld:pubmed
pubmed-article:17540587pubmed:publicationTypeJournal Articlelld:pubmed
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