| pubmed-article:17510178 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:17510178 | lifeskim:mentions | umls-concept:C0025914 | lld:lifeskim |
| pubmed-article:17510178 | lifeskim:mentions | umls-concept:C0026809 | lld:lifeskim |
| pubmed-article:17510178 | lifeskim:mentions | umls-concept:C1622418 | lld:lifeskim |
| pubmed-article:17510178 | lifeskim:mentions | umls-concept:C0015283 | lld:lifeskim |
| pubmed-article:17510178 | lifeskim:mentions | umls-concept:C1704259 | lld:lifeskim |
| pubmed-article:17510178 | lifeskim:mentions | umls-concept:C1705987 | lld:lifeskim |
| pubmed-article:17510178 | lifeskim:mentions | umls-concept:C0851285 | lld:lifeskim |
| pubmed-article:17510178 | pubmed:issue | Pt 3 | lld:pubmed |
| pubmed-article:17510178 | pubmed:dateCreated | 2007-8-3 | lld:pubmed |
| pubmed-article:17510178 | pubmed:abstractText | It has been reported that cAMP regulates Ca(2+)-dependent exocytosis via protein kinase A (PKA) and exchange proteins directly activated by cAMP (Epac) in neurons and secretory cells. It has, however, never been clarified how regulation of Ca(2+)-dependent exocytosis by cAMP differs depending on the involvement of PKA and Epac, and depending on two types of secretory vesicles, large dense-core vesicles (LVs) and small vesicles (SVs). In this study, we have directly visualized Ca(2+)-dependent exocytosis of both LVs and SVs with two-photon imaging in mouse pancreatic beta-cells. We found that marked exocytosis of SVs occurred with a time constant of 0.3 s, more than three times as fast as LV exocytosis, on stimulation by photolysis of a caged-Ca(2+) compound. The diameter of SVs was identified as approximately 80 nm with two-photon imaging, which was confirmed by electron-microscopic investigation with photoconversion of diaminobenzidine. Calcium-dependent exocytosis of SVs was potentiated by the cAMP-elevating agent forskolin, and the potentiating effect was unaffected by antagonists of PKA and was mimicked by the Epac-selective agonist 8-(4-chlorophenylthio)-2'-O-methyl cAMP, unlike that on LVs. Moreover, high-glucose stimulation induced massive exocytosis of SVs in addition to LVs, and photolysis of caged cAMP during glucose stimulation caused potentiation of exocytosis with little delay for SVs but with a latency of 5 s for LVs. Thus, Epac and PKA selectively regulate exocytosis of SVs and LVs, respectively, in beta-cells, and Epac can regulate exocytosis more rapidly than PKA. | lld:pubmed |
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| pubmed-article:17510178 | pubmed:language | eng | lld:pubmed |
| pubmed-article:17510178 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17510178 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:17510178 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17510178 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17510178 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17510178 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:17510178 | pubmed:month | Aug | lld:pubmed |
| pubmed-article:17510178 | pubmed:issn | 0022-3751 | lld:pubmed |
| pubmed-article:17510178 | pubmed:author | pubmed-author:KasaiHaruoH | lld:pubmed |
| pubmed-article:17510178 | pubmed:author | pubmed-author:KishimotoTaku... | lld:pubmed |
| pubmed-article:17510178 | pubmed:author | pubmed-author:TakahashiNori... | lld:pubmed |
| pubmed-article:17510178 | pubmed:author | pubmed-author:NemotoTomomiT | lld:pubmed |
| pubmed-article:17510178 | pubmed:author | pubmed-author:HatakeyamaHir... | lld:pubmed |
| pubmed-article:17510178 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:17510178 | pubmed:day | 1 | lld:pubmed |
| pubmed-article:17510178 | pubmed:volume | 582 | lld:pubmed |
| pubmed-article:17510178 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:17510178 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:17510178 | pubmed:pagination | 1087-98 | lld:pubmed |
| pubmed-article:17510178 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:17510178 | pubmed:meshHeading | pubmed-meshheading:17510178... | lld:pubmed |
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