| pubmed-article:17360403 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:17360403 | lifeskim:mentions | umls-concept:C0004651 | lld:lifeskim |
| pubmed-article:17360403 | lifeskim:mentions | umls-concept:C0600080 | lld:lifeskim |
| pubmed-article:17360403 | lifeskim:mentions | umls-concept:C1549810 | lld:lifeskim |
| pubmed-article:17360403 | lifeskim:mentions | umls-concept:C0205171 | lld:lifeskim |
| pubmed-article:17360403 | pubmed:issue | 12 | lld:pubmed |
| pubmed-article:17360403 | pubmed:dateCreated | 2007-3-21 | lld:pubmed |
| pubmed-article:17360403 | pubmed:abstractText | The ability to present biomolecules on the highly organized structure of M13 filamentous bacteriophage is a unique advantage. Where previously this viral template was shown to direct the orientation and nucleation of nanocrystals and materials, here we apply it in the context of single-molecule (SM) biophysics. Genetically engineered constructs were used to display different reactive species at each of the filament ends and along the major capsid, and the resulting hetero-functional particles were shown to consistently tether microscopic beads in solution. With this system, we report the development of a SM assay based on M13 bacteriophage. We also report the quantitative characterization of the biopolymer's elasticity by using an optical trap with nanometer-scale position resolution. Expanding the fluctuating rod limit of the wormlike chain to incorporate enthalpic polymer stretching yielded a model capable of accurately capturing the full range of extensions. Fits of the force-extension measurements gave a mean persistence length of approximately 1,265 nm, lending SM support for a shorter filamentous bacteriophage persistence length than previously thought. Furthermore, a predicted stretching modulus roughly two times that of dsDNA, coupled with the system's linkage versatility and load-bearing capability, makes the M13 template an attractive candidate for use in tethered bead architectures. | lld:pubmed |
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| pubmed-article:17360403 | pubmed:language | eng | lld:pubmed |
| pubmed-article:17360403 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17360403 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:17360403 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17360403 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:17360403 | pubmed:month | Mar | lld:pubmed |
| pubmed-article:17360403 | pubmed:issn | 0027-8424 | lld:pubmed |
| pubmed-article:17360403 | pubmed:author | pubmed-author:BelcherAngela... | lld:pubmed |
| pubmed-article:17360403 | pubmed:author | pubmed-author:LangMatthew... | lld:pubmed |
| pubmed-article:17360403 | pubmed:author | pubmed-author:NorenChristop... | lld:pubmed |
| pubmed-article:17360403 | pubmed:author | pubmed-author:FerrerJorge... | lld:pubmed |
| pubmed-article:17360403 | pubmed:author | pubmed-author:KottmannSteph... | lld:pubmed |
| pubmed-article:17360403 | pubmed:author | pubmed-author:BrauRicardo... | lld:pubmed |
| pubmed-article:17360403 | pubmed:author | pubmed-author:KhalilAhmad... | lld:pubmed |
| pubmed-article:17360403 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:17360403 | pubmed:day | 20 | lld:pubmed |
| pubmed-article:17360403 | pubmed:volume | 104 | lld:pubmed |
| pubmed-article:17360403 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:17360403 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:17360403 | pubmed:pagination | 4892-7 | lld:pubmed |
| pubmed-article:17360403 | pubmed:dateRevised | 2010-9-16 | lld:pubmed |
| pubmed-article:17360403 | pubmed:meshHeading | pubmed-meshheading:17360403... | lld:pubmed |
| pubmed-article:17360403 | pubmed:meshHeading | pubmed-meshheading:17360403... | lld:pubmed |
| pubmed-article:17360403 | pubmed:meshHeading | pubmed-meshheading:17360403... | lld:pubmed |
| pubmed-article:17360403 | pubmed:meshHeading | pubmed-meshheading:17360403... | lld:pubmed |
| pubmed-article:17360403 | pubmed:year | 2007 | lld:pubmed |
| pubmed-article:17360403 | pubmed:articleTitle | Single M13 bacteriophage tethering and stretching. | lld:pubmed |
| pubmed-article:17360403 | pubmed:affiliation | Department of Mechanical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA. | lld:pubmed |
| pubmed-article:17360403 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:17360403 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |
| pubmed-article:17360403 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| pubmed-article:17360403 | pubmed:publicationType | Research Support, N.I.H., Extramural | lld:pubmed |
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