| pubmed-article:17277069 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:17277069 | lifeskim:mentions | umls-concept:C0038174 | lld:lifeskim |
| pubmed-article:17277069 | lifeskim:mentions | umls-concept:C0033684 | lld:lifeskim |
| pubmed-article:17277069 | lifeskim:mentions | umls-concept:C1948066 | lld:lifeskim |
| pubmed-article:17277069 | lifeskim:mentions | umls-concept:C0392752 | lld:lifeskim |
| pubmed-article:17277069 | pubmed:issue | 7 | lld:pubmed |
| pubmed-article:17277069 | pubmed:dateCreated | 2007-3-19 | lld:pubmed |
| pubmed-article:17277069 | pubmed:abstractText | Staphylococcus epidermidis is both a human skin commensal and an opportunistic pathogen, causing infections linked to implanted medical devices. This paper describes localized tufts of fibrillar appendages on a subpopulation (25%) of wild-type (WT) S. epidermidis NCTC 11047 cells. The fibrils (122.2 +/- 10.8 nm long) are usually in a lateral position on the cells. Fibrillar (Fib(+)) and nonfibrillar (Fib(-)) subpopulations were separated (enriched) by 34 sequential partitions of WT cells between a buffer phase and a hexadecane phase. Following enrichment, hydrophobic cells from the hexadecane phase comprised 70% Fib(+) cells and the less hydrophobic cells from the buffer phase entirely comprised Fib(-) cells. The Fib(+) and Fib(-) subpopulations did not revert on subculture (34 times) on solid medium. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of cell surface proteins from WT, Fib(+), and Fib(-) cells revealed two high-molecular-mass proteins (280 kDa and 230 kDa) on the WT and Fib(+) cells that were absent from the Fib(-) cells. Amino acid sequencing revealed that fragments of both the 280- and 230-kDa proteins had 100% identity to the accumulation-associated protein (Aap). Aap is known to cause biofilm formation if it is truncated by loss of the terminal A domain. Immunogold staining with anti-Aap antibodies labeled tuft fibrils of the WT and Fib(+) cells but not the cell surface of Fib(-) cells. The tufts were labeled with N-terminally directed antibodies (anti-A domain), showing that the fibrillar Aap was not truncated on the cell surface. Thus, the presence of full-length Aap correlated with the low biofilm-forming abilities of both WT and Fib(+) S. epidermidis NCTC 11047 populations. Reverse transcription-PCR showed that aap was transcribed in both Fib(+) and Fib(-) cells. We therefore propose that full-length Aap is expressed on cells of S. epidermidis NCTC 11047 as tufts of short fibrils and that fibril expression is regulated at a posttranscriptional level. | lld:pubmed |
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| pubmed-article:17277069 | pubmed:language | eng | lld:pubmed |
| pubmed-article:17277069 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17277069 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:17277069 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:17277069 | pubmed:month | Apr | lld:pubmed |
| pubmed-article:17277069 | pubmed:issn | 0021-9193 | lld:pubmed |
| pubmed-article:17277069 | pubmed:author | pubmed-author:FosterTimothy... | lld:pubmed |
| pubmed-article:17277069 | pubmed:author | pubmed-author:MackDietrichD | lld:pubmed |
| pubmed-article:17277069 | pubmed:author | pubmed-author:RohdeHolgerH | lld:pubmed |
| pubmed-article:17277069 | pubmed:author | pubmed-author:HandleyPaulin... | lld:pubmed |
| pubmed-article:17277069 | pubmed:author | pubmed-author:UptonMathewM | lld:pubmed |
| pubmed-article:17277069 | pubmed:author | pubmed-author:BannerMiriam... | lld:pubmed |
| pubmed-article:17277069 | pubmed:author | pubmed-author:CunniffeJohn... | lld:pubmed |
| pubmed-article:17277069 | pubmed:author | pubmed-author:MacintoshRobi... | lld:pubmed |
| pubmed-article:17277069 | pubmed:author | pubmed-author:HoyesEmmyE | lld:pubmed |
| pubmed-article:17277069 | pubmed:author | pubmed-author:DerrickJeremy... | lld:pubmed |
| pubmed-article:17277069 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:17277069 | pubmed:volume | 189 | lld:pubmed |
| pubmed-article:17277069 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:17277069 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:17277069 | pubmed:pagination | 2793-804 | lld:pubmed |
| pubmed-article:17277069 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
| pubmed-article:17277069 | pubmed:meshHeading | pubmed-meshheading:17277069... | lld:pubmed |
| pubmed-article:17277069 | pubmed:meshHeading | pubmed-meshheading:17277069... | lld:pubmed |
| pubmed-article:17277069 | pubmed:meshHeading | pubmed-meshheading:17277069... | lld:pubmed |
| pubmed-article:17277069 | pubmed:meshHeading | pubmed-meshheading:17277069... | lld:pubmed |
| pubmed-article:17277069 | pubmed:meshHeading | pubmed-meshheading:17277069... | lld:pubmed |
| pubmed-article:17277069 | pubmed:meshHeading | pubmed-meshheading:17277069... | lld:pubmed |
| pubmed-article:17277069 | pubmed:meshHeading | pubmed-meshheading:17277069... | lld:pubmed |
| pubmed-article:17277069 | pubmed:meshHeading | pubmed-meshheading:17277069... | lld:pubmed |
| pubmed-article:17277069 | pubmed:year | 2007 | lld:pubmed |
| pubmed-article:17277069 | pubmed:articleTitle | Localized tufts of fibrils on Staphylococcus epidermidis NCTC 11047 are comprised of the accumulation-associated protein. | lld:pubmed |
| pubmed-article:17277069 | pubmed:affiliation | Faculty of Life Sciences, 1.800 Stopford Building, The University of Manchester, Oxford Road, Manchester M13 9PT, United Kingdom. | lld:pubmed |
| pubmed-article:17277069 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:17277069 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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