| pubmed-article:17245435 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:17245435 | lifeskim:mentions | umls-concept:C1705994 | lld:lifeskim |
| pubmed-article:17245435 | lifeskim:mentions | umls-concept:C1880202 | lld:lifeskim |
| pubmed-article:17245435 | lifeskim:mentions | umls-concept:C1513492 | lld:lifeskim |
| pubmed-article:17245435 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:17245435 | pubmed:dateCreated | 2007-1-24 | lld:pubmed |
| pubmed-article:17245435 | pubmed:abstractText | Direct imaging or counting of RNA molecules has been difficult owing to its relatively low electron density for EM and insufficient resolution in AFM. Bacteriophage phi29 DNA-packaging motor is geared by a packaging RNA (pRNA) ring. Currently, whether the ring is a pentagon or hexagon is under fervent debate. We report here the assembly of a highly sensitive imaging system for direct counting of the copy number of pRNA within this 20-nm motor. Single fluorophore imaging clearly identified the quantized photobleaching steps from pRNA labeled with a single fluorophore and concluded its stoichiometry within the motor. Almost all of the motors contained six copies of pRNA before and during DNA translocation, identified by dual-color detection of the stalled intermediates of motors containing Cy3-pRNA and Cy5-DNA. The stalled motors were restarted to observe the motion of DNA packaging in real time. Heat-denaturation analysis confirmed that the stoichiometry of pRNA is the common multiple of 2 and 3. EM imaging of procapsid/pRNA complexes clearly revealed six ferritin particles that were conjugated to each pRNA ring. | lld:pubmed |
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| pubmed-article:17245435 | pubmed:language | eng | lld:pubmed |
| pubmed-article:17245435 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17245435 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:17245435 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:17245435 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:17245435 | pubmed:month | Jan | lld:pubmed |
| pubmed-article:17245435 | pubmed:issn | 0261-4189 | lld:pubmed |
| pubmed-article:17245435 | pubmed:author | pubmed-author:ZhangHuiH | lld:pubmed |
| pubmed-article:17245435 | pubmed:author | pubmed-author:GuoPeixuanP | lld:pubmed |
| pubmed-article:17245435 | pubmed:author | pubmed-author:COXW FWF | lld:pubmed |
| pubmed-article:17245435 | pubmed:author | pubmed-author:JinJiashunJ | lld:pubmed |
| pubmed-article:17245435 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:17245435 | pubmed:day | 24 | lld:pubmed |
| pubmed-article:17245435 | pubmed:volume | 26 | lld:pubmed |
| pubmed-article:17245435 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:17245435 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:17245435 | pubmed:pagination | 527-37 | lld:pubmed |
| pubmed-article:17245435 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
| pubmed-article:17245435 | pubmed:meshHeading | pubmed-meshheading:17245435... | lld:pubmed |
| pubmed-article:17245435 | pubmed:meshHeading | pubmed-meshheading:17245435... | lld:pubmed |
| pubmed-article:17245435 | pubmed:meshHeading | pubmed-meshheading:17245435... | lld:pubmed |
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| pubmed-article:17245435 | pubmed:meshHeading | pubmed-meshheading:17245435... | lld:pubmed |
