| pubmed-article:17170521 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:17170521 | lifeskim:mentions | umls-concept:C0007634 | lld:lifeskim |
| pubmed-article:17170521 | lifeskim:mentions | umls-concept:C0034721 | lld:lifeskim |
| pubmed-article:17170521 | lifeskim:mentions | umls-concept:C0034693 | lld:lifeskim |
| pubmed-article:17170521 | lifeskim:mentions | umls-concept:C0221908 | lld:lifeskim |
| pubmed-article:17170521 | lifeskim:mentions | umls-concept:C0021853 | lld:lifeskim |
| pubmed-article:17170521 | lifeskim:mentions | umls-concept:C0013030 | lld:lifeskim |
| pubmed-article:17170521 | lifeskim:mentions | umls-concept:C0021467 | lld:lifeskim |
| pubmed-article:17170521 | lifeskim:mentions | umls-concept:C0441655 | lld:lifeskim |
| pubmed-article:17170521 | lifeskim:mentions | umls-concept:C1333700 | lld:lifeskim |
| pubmed-article:17170521 | lifeskim:mentions | umls-concept:C0021469 | lld:lifeskim |
| pubmed-article:17170521 | lifeskim:mentions | umls-concept:C1621574 | lld:lifeskim |
| pubmed-article:17170521 | lifeskim:mentions | umls-concept:C0851285 | lld:lifeskim |
| pubmed-article:17170521 | lifeskim:mentions | umls-concept:C0872383 | lld:lifeskim |
| pubmed-article:17170521 | pubmed:issue | 6 | lld:pubmed |
| pubmed-article:17170521 | pubmed:dateCreated | 2006-12-15 | lld:pubmed |
| pubmed-article:17170521 | pubmed:abstractText | The present study investigated the effect of dopamine D1-like receptor stimulation on the Cl-/HCO3- exchange activity in rat intestinal epithelial IEC-6 cells. The Cl-/HCO3- exchange activity was found to be a chloride-dependent, DIDS-sensitive and niflumate-insensitive process. The presence of the SLC26A6 anion exchanger was detected by both RT-PCR and immunoblotting analysis in IEC-6 cells, in which three different small interfering RNAs (siRNAs) targeting SLC26A6 markedly inhibited Cl-/HCO3- exchange. Activation of dopamine D1-like receptors with SKF 38393 inhibited Cl-/HCO3- exchanger activity, this being antagonized by the D1 selective antagonist SKF 83566. However, effects of SKF 38393 were maximal at 5 min of exposure to the agonist and rapidly diminished with no effect at 15 min, suggestive of agonist-induced desensitization of D1-like receptors. Pretreatment of cells with heparin, a non-selective inhibitor of G protein-coupled receptor kinases (GRKs), prevented the observed attenuation of SKF 38393-induced inhibition of Cl-/HCO3- exchange. Overnight pretreatment with anti-GRK6A and anti-GRK6B, but not with anti-GRK4 antibodies, prevented the loss of SKF 38393-mediated effects. Both PKA and PKC signaling pathways participate in SKF 38393-mediated inhibition of Cl-/HCO3- exchange. These findings suggest that SLC26A6 is at least one of the anion exchanger's family members responsible for Cl-/HCO3- exchange in IEC-6 cells. Dopamine D1 receptors in IEC-6 rapidly desensitize to D1-like agonist stimulation and GRK 6, but not GRK 4, appear to be involved in agonist-mediated responsiveness and desensitization. | lld:pubmed |
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| pubmed-article:17170521 | pubmed:language | eng | lld:pubmed |
| pubmed-article:17170521 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17170521 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:17170521 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:17170521 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:17170521 | pubmed:issn | 1015-8987 | lld:pubmed |
| pubmed-article:17170521 | pubmed:author | pubmed-author:MountDavid... | lld:pubmed |
| pubmed-article:17170521 | pubmed:author | pubmed-author:Soares-da-Sil... | lld:pubmed |
| pubmed-article:17170521 | pubmed:author | pubmed-author:FragaSóniaS | lld:pubmed |
| pubmed-article:17170521 | pubmed:author | pubmed-author:LuoYingjinY | lld:pubmed |
| pubmed-article:17170521 | pubmed:author | pubmed-author:Zandi-NejadKa... | lld:pubmed |
| pubmed-article:17170521 | pubmed:author | pubmed-author:JosePedroP | lld:pubmed |
| pubmed-article:17170521 | pubmed:copyrightInfo | Copyright 2006 S. Karger AG, Basel. | lld:pubmed |
| pubmed-article:17170521 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:17170521 | pubmed:volume | 18 | lld:pubmed |
| pubmed-article:17170521 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:17170521 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:17170521 | pubmed:pagination | 347-60 | lld:pubmed |
| pubmed-article:17170521 | pubmed:dateRevised | 2007-11-15 | lld:pubmed |
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| pubmed-article:17170521 | pubmed:year | 2006 | lld:pubmed |
| pubmed-article:17170521 | pubmed:articleTitle | Dopamine D1-like receptor-mediated inhibition of Cl/HCO3- exchanger activity in rat intestinal epithelial IEC-6 cells is regulated by G protein-coupled receptor kinase 6 (GRK 6). | lld:pubmed |
| pubmed-article:17170521 | pubmed:affiliation | Institute of Pharmacology and Therapeutics, Faculty of Medicine, Porto, Portugal. | lld:pubmed |
| pubmed-article:17170521 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:17170521 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |
| pubmed-article:17170521 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| pubmed-article:17170521 | pubmed:publicationType | Research Support, N.I.H., Extramural | lld:pubmed |
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