rdf:type |
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lifeskim:mentions |
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pubmed:issue |
24
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pubmed:dateCreated |
2006-12-13
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pubmed:abstractText |
Sphingosine 1-phosphate (S1P), produced by sphingosine kinase (SPHK), acts both by intracellular and extracellular modes. We evaluated the role of SPHK1 and S1P in osteoclastogenesis using bone marrow-derived macrophage (BMM) single and BMM/osteoblast coculture systems. In BMM single cultures, the osteoclastogenic factor receptor activator of NF-kappaB ligand (RANKL) upregulated SPHK1 and increased S1P production and secretion. SPHK1 siRNA enhanced and SPHK1 overexpression attenuated osteoclastogenesis via modulation of p38 and ERK activities, and NFATc1 and c-Fos levels. Extracellular S1P had no effect in these cultures. These data suggest that intracellular S1P produced in response to RANKL forms a negative feedback loop in BMM single cultures. In contrast, S1P addition to BMM/osteoblast cocultures greatly increased osteoclastogenesis by increasing RANKL in osteoblasts via cyclooxygenase-2 and PGE(2) regulation. S1P also stimulated osteoblast migration and survival. The RANKL elevation and chemotactic effects were also observed with T cells. These results indicate that secreted S1P attracts and acts on osteoblasts and T cells to augment osteoclastogenesis. Taken together, S1P plays an important role in osteoclastogenesis regulation and in communication between osteoclasts and osteoblasts or T cells.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/17124500-10215628,
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Cyclooxygenase 2,
http://linkedlifedata.com/resource/pubmed/chemical/Dinoprostone,
http://linkedlifedata.com/resource/pubmed/chemical/Extracellular Signal-Regulated MAP...,
http://linkedlifedata.com/resource/pubmed/chemical/Lysophospholipids,
http://linkedlifedata.com/resource/pubmed/chemical/NFATC Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphotransferases (Alcohol Group...,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-fos,
http://linkedlifedata.com/resource/pubmed/chemical/RANK Ligand,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Sphingosine,
http://linkedlifedata.com/resource/pubmed/chemical/p38 Mitogen-Activated Protein...,
http://linkedlifedata.com/resource/pubmed/chemical/sphingosine 1-phosphate,
http://linkedlifedata.com/resource/pubmed/chemical/sphingosine kinase
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0261-4189
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
13
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pubmed:volume |
25
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
5840-51
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:17124500-Animals,
pubmed-meshheading:17124500-Cell Differentiation,
pubmed-meshheading:17124500-Cell Survival,
pubmed-meshheading:17124500-Chemotaxis,
pubmed-meshheading:17124500-Coculture Techniques,
pubmed-meshheading:17124500-Cyclooxygenase 2,
pubmed-meshheading:17124500-Dinoprostone,
pubmed-meshheading:17124500-Extracellular Signal-Regulated MAP Kinases,
pubmed-meshheading:17124500-Gene Expression Regulation, Enzymologic,
pubmed-meshheading:17124500-HeLa Cells,
pubmed-meshheading:17124500-Humans,
pubmed-meshheading:17124500-Lymphocyte Activation,
pubmed-meshheading:17124500-Lysophospholipids,
pubmed-meshheading:17124500-Mice,
pubmed-meshheading:17124500-NFATC Transcription Factors,
pubmed-meshheading:17124500-Osteoblasts,
pubmed-meshheading:17124500-Osteoclasts,
pubmed-meshheading:17124500-Osteogenesis,
pubmed-meshheading:17124500-Phosphotransferases (Alcohol Group Acceptor),
pubmed-meshheading:17124500-Proto-Oncogene Proteins c-fos,
pubmed-meshheading:17124500-RANK Ligand,
pubmed-meshheading:17124500-RNA, Messenger,
pubmed-meshheading:17124500-Sphingosine,
pubmed-meshheading:17124500-T-Lymphocytes,
pubmed-meshheading:17124500-p38 Mitogen-Activated Protein Kinases
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pubmed:year |
2006
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pubmed:articleTitle |
Sphingosine 1-phosphate as a regulator of osteoclast differentiation and osteoclast-osteoblast coupling.
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pubmed:affiliation |
Department of Cell and Developmental Biology, BK21 Program, DRI, Seoul National University, 28 Yeongon-Dong, Chongno-Gu, Seoul 110-749, Korea.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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