| pubmed-article:1707813 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:1707813 | lifeskim:mentions | umls-concept:C0002892 | lld:lifeskim |
| pubmed-article:1707813 | lifeskim:mentions | umls-concept:C1704242 | lld:lifeskim |
| pubmed-article:1707813 | lifeskim:mentions | umls-concept:C0003250 | lld:lifeskim |
| pubmed-article:1707813 | lifeskim:mentions | umls-concept:C0033684 | lld:lifeskim |
| pubmed-article:1707813 | lifeskim:mentions | umls-concept:C0018440 | lld:lifeskim |
| pubmed-article:1707813 | lifeskim:mentions | umls-concept:C0001473 | lld:lifeskim |
| pubmed-article:1707813 | lifeskim:mentions | umls-concept:C0004359 | lld:lifeskim |
| pubmed-article:1707813 | lifeskim:mentions | umls-concept:C0205369 | lld:lifeskim |
| pubmed-article:1707813 | lifeskim:mentions | umls-concept:C0444669 | lld:lifeskim |
| pubmed-article:1707813 | lifeskim:mentions | umls-concept:C1521840 | lld:lifeskim |
| pubmed-article:1707813 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:1707813 | pubmed:dateCreated | 1991-5-23 | lld:pubmed |
| pubmed-article:1707813 | pubmed:abstractText | The gastric H+/K(+)-transporting adenosine triphosphatase (H+/K+ ATPase) (proton pump) consists of a catalytic alpha-subunit and a recently proposed 60-90-kDa glycoprotein beta-subunit. Using dog gastric membranes as the antigen, we have produced two murine monoclonal antibodies, 4F11 (IgG1) and 3A6 (IgA), which are specific for the 60-90-kDa glycoprotein. The monoclonal antibodies (1) specifically stained the cytoplasm of unfixed and formalin-fixed dog gastric parietal cells; (2) specifically reacted by ELISA with gastric tubulovesicular membranes; (3) recognised epitopes located on the luminal face of parietal cell tubulovesicular membranes, the site of the proton pump, by immunogold electron microscopy; (4) immunoblotted a 60-90-kDa molecule from tubulovesicular membranes and a 35-kDa component from peptide N-glycosidase-F-treated membrane extracts; (5) immunoblotted the 60-90-kDa parietal cell autoantigen associated with autoimmune gastritis and pernicious anemia, purified by chromatography on parietal cell autoantibody- or tomato-lectin-Sepharose 4B affinity columns, and the 35-kDa protein core of this autoantigen; this autoantigen has amino acid sequence similarity to the beta-subunit of the related Na+/K(+)-transporting adenosine triphosphatase (Na+/K+ ATPase) [Toh et al. (1990) Proc. Natl Acad. Sci. 87, 6418-6422]; (6) co-precipitated a molecule of 95 kDa with the 60-90-kDa molecule from 125I-labelled detergent extracts of dog tubulovesicular membranes; and (7) co-purified the catalytic alpha-subunit of the H+/K+ ATPase with the 60-90-kDa molecule by immunoaffinity chromatography of tubulovesicular membrane extracts on a monoclonal antibody 3A6-Sepharose 4B column, indicating a physical association between the two molecules. These results provide further evidence that the 60-90-kDa glycoprotein is the beta-subunit of the gastric H+/K+ ATPase. We conclude that the monoclonal antibodies specifically recognise luminal epitopes on the 35-kDa core protein of the 60-90-kDa beta-subunit of the gastric proton pump, a major target molecule in autoimmune gastritis and pernicious anaemia. These monoclonal antibodies will be valuable probes to study the structure and function of this associated beta-subunit, as well as the ontogeny of the gastric proton pump. | lld:pubmed |
| pubmed-article:1707813 | pubmed:language | eng | lld:pubmed |
| pubmed-article:1707813 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1707813 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:1707813 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:1707813 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1707813 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1707813 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1707813 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1707813 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1707813 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:1707813 | pubmed:month | Apr | lld:pubmed |
| pubmed-article:1707813 | pubmed:issn | 0014-2956 | lld:pubmed |
| pubmed-article:1707813 | pubmed:author | pubmed-author:TohB HBH | lld:pubmed |
| pubmed-article:1707813 | pubmed:author | pubmed-author:JonesC MCM | lld:pubmed |
| pubmed-article:1707813 | pubmed:author | pubmed-author:GleesonP APA | lld:pubmed |
| pubmed-article:1707813 | pubmed:author | pubmed-author:PettittJ MJM | lld:pubmed |
| pubmed-article:1707813 | pubmed:author | pubmed-author:CallaghanJ... | lld:pubmed |
| pubmed-article:1707813 | pubmed:author | pubmed-author:HumphrisD CDC | lld:pubmed |
| pubmed-article:1707813 | pubmed:author | pubmed-author:MuF TFT | lld:pubmed |
| pubmed-article:1707813 | pubmed:author | pubmed-author:GoldkornII | lld:pubmed |
| pubmed-article:1707813 | pubmed:author | pubmed-author:MartinelliT... | lld:pubmed |
| pubmed-article:1707813 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:1707813 | pubmed:day | 10 | lld:pubmed |
| pubmed-article:1707813 | pubmed:volume | 197 | lld:pubmed |
| pubmed-article:1707813 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:1707813 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:1707813 | pubmed:pagination | 49-59 | lld:pubmed |
| pubmed-article:1707813 | pubmed:dateRevised | 2007-7-23 | lld:pubmed |
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| pubmed-article:1707813 | pubmed:year | 1991 | lld:pubmed |
| pubmed-article:1707813 | pubmed:articleTitle | Monoclonal antibodies specific for the core protein of the beta-subunit of the gastric proton pump (H+/K+ ATPase). An autoantigen targetted in pernicious anaemia. | lld:pubmed |
| pubmed-article:1707813 | pubmed:affiliation | Department of Pathology and Immunology, Monash University Medical School, Melbourne, Australia. | lld:pubmed |
| pubmed-article:1707813 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:1707813 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1707813 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1707813 | lld:pubmed |
