| pubmed-article:16839456 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:16839456 | lifeskim:mentions | umls-concept:C0034721 | lld:lifeskim |
| pubmed-article:16839456 | lifeskim:mentions | umls-concept:C0034693 | lld:lifeskim |
| pubmed-article:16839456 | lifeskim:mentions | umls-concept:C2339371 | lld:lifeskim |
| pubmed-article:16839456 | lifeskim:mentions | umls-concept:C0025979 | lld:lifeskim |
| pubmed-article:16839456 | lifeskim:mentions | umls-concept:C0750572 | lld:lifeskim |
| pubmed-article:16839456 | lifeskim:mentions | umls-concept:C0596235 | lld:lifeskim |
| pubmed-article:16839456 | lifeskim:mentions | umls-concept:C1524063 | lld:lifeskim |
| pubmed-article:16839456 | lifeskim:mentions | umls-concept:C0205134 | lld:lifeskim |
| pubmed-article:16839456 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:16839456 | pubmed:dateCreated | 2006-9-15 | lld:pubmed |
| pubmed-article:16839456 | pubmed:abstractText | We previously estimated the myofilament responsiveness to Ca(2+) in isolated intact ventricular myocytes, using the steady-state relationship between cytosolic Ca(2+) concentration ([Ca(2+)](i)) and cell-shortening during tetanus (Ca-L trajectory). This method was useful and easy; however, it could not be used for a high dose of Ca sensitizer because the instantaneous plots after the application of Ca sensitizer did not make a fixed point of shortening (we used 5% shortening). Therefore we must produce another method to investigate Ca(2+) responsiveness. For an estimation of a wider range of the Ca-L trajectory, we fitted the Ca-L trajectory data with the Hill equation to construct the Ca-shortening curve. To fit this curve, we measured the maximal shortening, which was on average 31.6%. The value of [Ca(2+)](i) to produce the half-maximal shortening (Ca(50)) was dose-dependently decreased by EMD57033 (sensitization). Either isoproterenol or 3-isobutyl-1-methylxanthine increased Ca(50) (desensitization) with a concomitant increase in intracellular c-AMP. EMD57439, a selective PDE-III inhibitor, did not significantly increase the c-AMP concentration and produced little change in Ca(50). These results are in agreement with previous reports with skinned or intact multicellular preparations. The Ca-shortening curve constructed in intact cardiac myocytes can be used to estimate the myofibrillar responsiveness to Ca(2+) in a wide range of [Ca(2+)](i). | lld:pubmed |
| pubmed-article:16839456 | pubmed:language | eng | lld:pubmed |
| pubmed-article:16839456 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16839456 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:16839456 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16839456 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16839456 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16839456 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16839456 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16839456 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16839456 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16839456 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16839456 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16839456 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:16839456 | pubmed:month | Jun | lld:pubmed |
| pubmed-article:16839456 | pubmed:issn | 1880-6546 | lld:pubmed |
| pubmed-article:16839456 | pubmed:author | pubmed-author:KuriharaSatos... | lld:pubmed |
| pubmed-article:16839456 | pubmed:author | pubmed-author:KawaiMakotoM | lld:pubmed |
| pubmed-article:16839456 | pubmed:author | pubmed-author:HongoKenichiK | lld:pubmed |
| pubmed-article:16839456 | pubmed:author | pubmed-author:KusakariYoich... | lld:pubmed |
| pubmed-article:16839456 | pubmed:author | pubmed-author:KonishiMasato... | lld:pubmed |
| pubmed-article:16839456 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:16839456 | pubmed:volume | 56 | lld:pubmed |
| pubmed-article:16839456 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:16839456 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:16839456 | pubmed:pagination | 219-26 | lld:pubmed |
| pubmed-article:16839456 | pubmed:dateRevised | 2011-11-17 | lld:pubmed |
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| pubmed-article:16839456 | pubmed:meshHeading | pubmed-meshheading:16839456... | lld:pubmed |
| pubmed-article:16839456 | pubmed:year | 2006 | lld:pubmed |
| pubmed-article:16839456 | pubmed:articleTitle | Use of the Ca-shortening curve to estimate the myofilament responsiveness to Ca2+ in tetanized rat ventricular myocytes. | lld:pubmed |
| pubmed-article:16839456 | pubmed:affiliation | Department of Physiology (II), The Jikei University School of Medicine, 3-25-8 Nishishinbashi, Minato-ku, Tokyo 105-8461, Japan. kusakari@jikei.ac.jp | lld:pubmed |
| pubmed-article:16839456 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:16839456 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
