| pubmed-article:16807119 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:16807119 | lifeskim:mentions | umls-concept:C0001483 | lld:lifeskim |
| pubmed-article:16807119 | lifeskim:mentions | umls-concept:C0007634 | lld:lifeskim |
| pubmed-article:16807119 | lifeskim:mentions | umls-concept:C0282641 | lld:lifeskim |
| pubmed-article:16807119 | lifeskim:mentions | umls-concept:C0017337 | lld:lifeskim |
| pubmed-article:16807119 | lifeskim:mentions | umls-concept:C1274040 | lld:lifeskim |
| pubmed-article:16807119 | lifeskim:mentions | umls-concept:C0205217 | lld:lifeskim |
| pubmed-article:16807119 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:16807119 | lifeskim:mentions | umls-concept:C0205148 | lld:lifeskim |
| pubmed-article:16807119 | lifeskim:mentions | umls-concept:C0086022 | lld:lifeskim |
| pubmed-article:16807119 | lifeskim:mentions | umls-concept:C1450054 | lld:lifeskim |
| pubmed-article:16807119 | lifeskim:mentions | umls-concept:C1550548 | lld:lifeskim |
| pubmed-article:16807119 | lifeskim:mentions | umls-concept:C1555714 | lld:lifeskim |
| pubmed-article:16807119 | lifeskim:mentions | umls-concept:C1705654 | lld:lifeskim |
| pubmed-article:16807119 | lifeskim:mentions | umls-concept:C1705099 | lld:lifeskim |
| pubmed-article:16807119 | lifeskim:mentions | umls-concept:C0442335 | lld:lifeskim |
| pubmed-article:16807119 | lifeskim:mentions | umls-concept:C2911684 | lld:lifeskim |
| pubmed-article:16807119 | lifeskim:mentions | umls-concept:C0185117 | lld:lifeskim |
| pubmed-article:16807119 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:16807119 | pubmed:dateCreated | 2006-8-14 | lld:pubmed |
| pubmed-article:16807119 | pubmed:abstractText | The present studies investigated the hypothesis that affinity immobilization of replication-defective adenoviruses (Ad) on the surfaces of biodegradable nanoparticles (NP) can improve transduction through uncoupling cellular uptake from the coxsackie-adenovirus receptor (CAR). Ad was tethered to the surfaces of polylactide-based NP that were surface-activated using a photoreactive polyallylamine-benzophenone-pyridyldithiocarboxylate polymer, which enabled (via thiol chemistry) the covalent attachment of Ad-binding proteins, either the recombinant D1 domain of CAR or an adenoviral knob-specific monoclonal antibody. Gene transfer by NP-Ad complexes was studied in relation to cellular uptake as a function of cell type and the character of NP-Ad binding. NP-Ad complexes, but not Ad applied with or without control nonimmune IgG-modified NP, significantly increased green fluorescent protein reporter expression in endothelioma and endothelial and arterial smooth muscle cells (SMC) in direct correlation to the extent of NP-Ad internalization. CAR-independent uptake of NP-Ad was confirmed by demonstrating inhibition of free Ad- but not NP-Ad complex-mediated transduction by knob protein. Complexes formulated with an Ad encoding inducible nitric oxide synthase inhibited growth of cultured SMC to a significantly greater extent than those with (GFP)Ad or (NULL)Ad or free vector. It is concluded that Ad-specific affinity tethering to biodegradable NP can significantly increase the level of gene expression via a CAR-independent uptake mechanism. | lld:pubmed |
| pubmed-article:16807119 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16807119 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16807119 | pubmed:language | eng | lld:pubmed |
| pubmed-article:16807119 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16807119 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:16807119 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16807119 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16807119 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16807119 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16807119 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16807119 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16807119 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16807119 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16807119 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16807119 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:16807119 | pubmed:month | Sep | lld:pubmed |
| pubmed-article:16807119 | pubmed:issn | 1525-0016 | lld:pubmed |
| pubmed-article:16807119 | pubmed:author | pubmed-author:LevyRobert... | lld:pubmed |
| pubmed-article:16807119 | pubmed:author | pubmed-author:FishbeinIliaI | lld:pubmed |
| pubmed-article:16807119 | pubmed:author | pubmed-author:AlferievIvan... | lld:pubmed |
| pubmed-article:16807119 | pubmed:author | pubmed-author:ChornyMichael... | lld:pubmed |
| pubmed-article:16807119 | pubmed:author | pubmed-author:GasterRichard... | lld:pubmed |
| pubmed-article:16807119 | pubmed:author | pubmed-author:NyanguileOrig... | lld:pubmed |
| pubmed-article:16807119 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:16807119 | pubmed:volume | 14 | lld:pubmed |
| pubmed-article:16807119 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:16807119 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:16807119 | pubmed:pagination | 382-91 | lld:pubmed |
| pubmed-article:16807119 | pubmed:dateRevised | 2011-7-1 | lld:pubmed |
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| pubmed-article:16807119 | pubmed:meshHeading | pubmed-meshheading:16807119... | lld:pubmed |
| pubmed-article:16807119 | pubmed:meshHeading | pubmed-meshheading:16807119... | lld:pubmed |
| pubmed-article:16807119 | pubmed:meshHeading | pubmed-meshheading:16807119... | lld:pubmed |
| pubmed-article:16807119 | pubmed:meshHeading | pubmed-meshheading:16807119... | lld:pubmed |
| pubmed-article:16807119 | pubmed:meshHeading | pubmed-meshheading:16807119... | lld:pubmed |
| pubmed-article:16807119 | pubmed:meshHeading | pubmed-meshheading:16807119... | lld:pubmed |
| pubmed-article:16807119 | pubmed:year | 2006 | lld:pubmed |
| pubmed-article:16807119 | pubmed:articleTitle | Adenoviral gene vector tethering to nanoparticle surfaces results in receptor-independent cell entry and increased transgene expression. | lld:pubmed |
| pubmed-article:16807119 | pubmed:affiliation | Division of Cardiology, The Children's Hospital of Philadelphia, Philadelphia, PA 19104-4318, USA. | lld:pubmed |
| pubmed-article:16807119 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:16807119 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| pubmed-article:16807119 | pubmed:publicationType | Research Support, N.I.H., Extramural | lld:pubmed |
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| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:16807119 | lld:pubmed |
