| pubmed-article:16801425 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:16801425 | lifeskim:mentions | umls-concept:C0038172 | lld:lifeskim |
| pubmed-article:16801425 | lifeskim:mentions | umls-concept:C0005821 | lld:lifeskim |
| pubmed-article:16801425 | lifeskim:mentions | umls-concept:C0033684 | lld:lifeskim |
| pubmed-article:16801425 | lifeskim:mentions | umls-concept:C0017337 | lld:lifeskim |
| pubmed-article:16801425 | lifeskim:mentions | umls-concept:C0332281 | lld:lifeskim |
| pubmed-article:16801425 | lifeskim:mentions | umls-concept:C0683598 | lld:lifeskim |
| pubmed-article:16801425 | lifeskim:mentions | umls-concept:C1153516 | lld:lifeskim |
| pubmed-article:16801425 | lifeskim:mentions | umls-concept:C1533691 | lld:lifeskim |
| pubmed-article:16801425 | lifeskim:mentions | umls-concept:C0332291 | lld:lifeskim |
| pubmed-article:16801425 | pubmed:issue | 7 | lld:pubmed |
| pubmed-article:16801425 | pubmed:dateCreated | 2006-6-27 | lld:pubmed |
| pubmed-article:16801425 | pubmed:abstractText | Thrombin-induced platelet microbial protein 1 (tPMP-1), a cationic antimicrobial polypeptide released from thrombin-stimulated rabbit platelets, targets the Staphylococcus aureus cytoplasmic membrane to initiate its microbicidal effects. In vitro resistance to tPMP-1 correlates with survival advantages in vivo. In S. aureus, the plasmid-carried qacA gene encodes a multidrug transporter, conferring resistance to organic cations (e.g., ethidium [Et]) via proton motive force (PMF)-energized export. We previously showed that qacA also confers a tPMP-1-resistant (tPMP-1r) phenotype in vitro. The current study evaluated whether (i) transporters encoded by the qacB and qacC multidrug resistance genes also confer tPMP-1r and (ii) tPMP-1r mediated by qacA is dependent on efflux pump activity. In contrast to tPMP-1r qacA-bearing strains, the parental strain and its isogenic qacB- and qacC-containing strains were tPMP-1 susceptible (tPMP-1s). Efflux pump inhibition by cyanide m-chlorophenylhydrazone abrogated Etr, but not tPMP-1r, in the qacA-bearing strain. In synergy assays, exposure of the qacA-bearing strain to tPMP-1 did not affect the susceptibility of Et (ruling out Et-tPMP-1 cotransport). The following cytoplasmic membrane parameters did not differ significantly between the qacA-bearing and parental strains: contents of the major phospholipids; asymmetric distributions of the positively charged species, lysyl-phosphotidylglycerol; fatty acid composition; and relative surface charge. Of note, the qacA-bearing strain exhibited greater membrane fluidity than that of the parental, qacB-, or qacC-bearing strain. In conclusion, among these families of efflux pumps, only the multidrug transporter encoded by qacA conferred a tPMP-1r phenotype. These data suggest that qacA-encoded tPMP-1r results from the impact of a specific transporter upon membrane structure or function unrelated to PMF-dependent peptide efflux. | lld:pubmed |
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| pubmed-article:16801425 | pubmed:language | eng | lld:pubmed |
| pubmed-article:16801425 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16801425 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:16801425 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:16801425 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:16801425 | pubmed:month | Jul | lld:pubmed |
| pubmed-article:16801425 | pubmed:issn | 0066-4804 | lld:pubmed |
| pubmed-article:16801425 | pubmed:author | pubmed-author:JonesTT | lld:pubmed |
| pubmed-article:16801425 | pubmed:author | pubmed-author:BayerA SAS | lld:pubmed |
| pubmed-article:16801425 | pubmed:author | pubmed-author:BrownM HMH | lld:pubmed |
| pubmed-article:16801425 | pubmed:author | pubmed-author:SkurrayR ARA | lld:pubmed |
| pubmed-article:16801425 | pubmed:author | pubmed-author:YeamanM RMR | lld:pubmed |
| pubmed-article:16801425 | pubmed:author | pubmed-author:GrkovicSS | lld:pubmed |
| pubmed-article:16801425 | pubmed:author | pubmed-author:KupferwasserL... | lld:pubmed |
| pubmed-article:16801425 | pubmed:author | pubmed-author:MukhopadhayKK | lld:pubmed |
| pubmed-article:16801425 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:16801425 | pubmed:volume | 50 | lld:pubmed |
| pubmed-article:16801425 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:16801425 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:16801425 | pubmed:pagination | 2448-54 | lld:pubmed |
| pubmed-article:16801425 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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