Linked Life Data

16728405

Source:http://linkedlifedata.com/resource/pubmed/id/16728405

Statements in which the resource exists.
SubjectPredicateObjectContext
pubmed-article:16728405rdf:typepubmed:Citationlld:pubmed
pubmed-article:16728405lifeskim:mentionsumls-concept:C0282635lld:lifeskim
pubmed-article:16728405lifeskim:mentionsumls-concept:C0996529lld:lifeskim
pubmed-article:16728405lifeskim:mentionsumls-concept:C0020792lld:lifeskim
pubmed-article:16728405lifeskim:mentionsumls-concept:C0017337lld:lifeskim
pubmed-article:16728405lifeskim:mentionsumls-concept:C0000545lld:lifeskim
pubmed-article:16728405lifeskim:mentionsumls-concept:C0205245lld:lifeskim
pubmed-article:16728405lifeskim:mentionsumls-concept:C0220781lld:lifeskim
pubmed-article:16728405lifeskim:mentionsumls-concept:C1314939lld:lifeskim
pubmed-article:16728405lifeskim:mentionsumls-concept:C1880022lld:lifeskim
pubmed-article:16728405pubmed:issue31lld:pubmed
pubmed-article:16728405pubmed:dateCreated2006-7-31lld:pubmed
pubmed-article:16728405pubmed:abstractTextThe moss Physcomitrella patens contains high levels of arachidonic acid and lesser amounts of eicosapentaenoic acid. Here we report the identification and characterization of a delta5-desaturase from P. patens that is associated with the synthesis of these fatty acids. A full-length cDNA for this desaturase was identified by data base searches based on homology to sequences of known delta5-desaturase cDNAs from fungal and algal species. The resulting P. patens cDNA encodes a 480-amino acid polypeptide that contains a predicted N-terminal cytochrome b5-like domain as well as three histidine-rich domains. Expression of the enzyme in Saccharomyces cerevisiae resulted in the production of the delta5-containing fatty acid arachidonic acid in cells that were provided di-homo-gamma-linolenic acid. In addition, the expressed enzyme generated delta5-desaturation products with the C20 substrates omega-6 eicosadienoic and omega-3 eicosatrienoic acids, but no products were detected with the C18 fatty acid linoleic and alpha-linolenic acids or with the C22 fatty acid adrenic and docosapentaenoic acids. When the corresponding P. patens genomic sequence was disrupted by replacement through homologous recombination, a dramatic alteration in the fatty acid composition was observed, i.e. an increase in di-homo-gamma-linolenic and eicosatetraenoic acids accompanied by a concomitant disappearance of the delta5-fatty acid arachidonic and eicosapentaenoic acids. In addition, overexpression of the P. patens cDNA in protoplasts isolated from a disrupted line resulted in the restoration of arachidonic acid synthesis.lld:pubmed
pubmed-article:16728405pubmed:languageenglld:pubmed
pubmed-article:16728405pubmed:journalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:16728405pubmed:citationSubsetIMlld:pubmed
pubmed-article:16728405pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:16728405pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:16728405pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:16728405pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:16728405pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:16728405pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:16728405pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:16728405pubmed:statusMEDLINElld:pubmed
pubmed-article:16728405pubmed:monthAuglld:pubmed
pubmed-article:16728405pubmed:issn0021-9258lld:pubmed
pubmed-article:16728405pubmed:authorpubmed-author:WiwatChanpenClld:pubmed
pubmed-article:16728405pubmed:authorpubmed-author:CahoonEdgar...lld:pubmed
pubmed-article:16728405pubmed:authorpubmed-author:QuatranoRalph...lld:pubmed
pubmed-article:16728405pubmed:authorpubmed-author:CoveDavid JDJlld:pubmed
pubmed-article:16728405pubmed:authorpubmed-author:Bunyapraphats...lld:pubmed
pubmed-article:16728405pubmed:authorpubmed-author:PerroudPierre...lld:pubmed
pubmed-article:16728405pubmed:authorpubmed-author:KaewsuwanSong...lld:pubmed
pubmed-article:16728405pubmed:authorpubmed-author:PanvisavasNat...lld:pubmed
pubmed-article:16728405pubmed:issnTypePrintlld:pubmed
pubmed-article:16728405pubmed:day4lld:pubmed
pubmed-article:16728405pubmed:volume281lld:pubmed
pubmed-article:16728405pubmed:ownerNLMlld:pubmed
pubmed-article:16728405pubmed:authorsCompleteYlld:pubmed
pubmed-article:16728405pubmed:pagination21988-97lld:pubmed
pubmed-article:16728405pubmed:dateRevised2009-11-19lld:pubmed
pubmed-article:16728405pubmed:meshHeadingpubmed-meshheading:16728405...lld:pubmed
pubmed-article:16728405pubmed:meshHeadingpubmed-meshheading:16728405...lld:pubmed
pubmed-article:16728405pubmed:meshHeadingpubmed-meshheading:16728405...lld:pubmed
pubmed-article:16728405pubmed:meshHeadingpubmed-meshheading:16728405...lld:pubmed
pubmed-article:16728405pubmed:meshHeadingpubmed-meshheading:16728405...lld:pubmed
pubmed-article:16728405pubmed:meshHeadingpubmed-meshheading:16728405...lld:pubmed
pubmed-article:16728405pubmed:meshHeadingpubmed-meshheading:16728405...lld:pubmed
pubmed-article:16728405pubmed:meshHeadingpubmed-meshheading:16728405...lld:pubmed
pubmed-article:16728405pubmed:meshHeadingpubmed-meshheading:16728405...lld:pubmed
pubmed-article:16728405pubmed:meshHeadingpubmed-meshheading:16728405...lld:pubmed
pubmed-article:16728405pubmed:year2006lld:pubmed
pubmed-article:16728405pubmed:articleTitleIdentification and functional characterization of the moss Physcomitrella patens delta5-desaturase gene involved in arachidonic and eicosapentaenoic acid biosynthesis.lld:pubmed
pubmed-article:16728405pubmed:affiliationDepartment of Pharmacognosy, Faculty of Pharmacy, Mahidol University, 447 Sri-Ayudhya Road, Ratchathewi, Bangkok 10400, Thailand.lld:pubmed
pubmed-article:16728405pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:16728405pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed
entrez-gene:1102486entrezgene:pubmedpubmed-article:16728405lld:entrezgene
entrez-gene:1099118entrezgene:pubmedpubmed-article:16728405lld:entrezgene
http://linkedlifedata.com/r...entrezgene:pubmedpubmed-article:16728405lld:entrezgene
http://linkedlifedata.com/r...entrezgene:pubmedpubmed-article:16728405lld:entrezgene
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:16728405lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:16728405lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:16728405lld:pubmed