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pubmed-article:16665435pubmed:abstractTextEtiolated Avena sativa L. seedlings grown in the presence of gabaculine (5-amino-1,3-cyclohexadienylcarboxylic acid) contained reduced levels of phytochrome as shown by spectrophotometric and immunochemical assays. Photochromic phytochrome levels in gabaculine-grown plants were estimated to be 20% of control plants, while immunoblot analysis showed that the phytochrome protein moiety was present at approximately 50% of control levels. Gabaculine-grown seedlings administered either 5-aminolevulinic acid or biliverdin exhibited a rapid increase of spectrophotometrically detectable phytochrome. Phytochrome concentrations estimated immunochemically did not similarly increase throughout treatment with either compound. Similar experiments with 5-amino[4-(14)C] levulinic acid showed radiolabeling of phytochrome with kinetics that paralleled the spectrally detected increase. These results are consistent with (a) the intermediacy of both 5-aminolevulinic acid and biliverdin in the biosynthetic pathway of the phytochrome chromophore and (b) the lack of coordinate regulation of chromophore and apoprotein synthesis in Avena seedlings.lld:pubmed
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pubmed-article:16665435pubmed:authorpubmed-author:LagariasJ CJClld:pubmed
pubmed-article:16665435pubmed:authorpubmed-author:ElichT DTDlld:pubmed
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pubmed-article:16665435pubmed:pagination304-10lld:pubmed
pubmed-article:16665435pubmed:dateRevised2010-9-15lld:pubmed
pubmed-article:16665435pubmed:year1987lld:pubmed
pubmed-article:16665435pubmed:articleTitlePhytochrome Chromophore Biosynthesis : Both 5-Aminolevulinic Acid and Biliverdin Overcome Inhibition by Gabaculine in Etiolated Avena sativa L. Seedlings.lld:pubmed
pubmed-article:16665435pubmed:affiliationDepartment of Biochemistry and Biophysics, University of California, Davis, California 95616.lld:pubmed
pubmed-article:16665435pubmed:publicationTypeJournal Articlelld:pubmed
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