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pubmed-article:16640731pubmed:abstractTextJuvenile hormone III (JH III) stimulates biosynthesis of the monoterpenoid aggregation pheromone component, ipsdienol, in the anterior midgut of the male pine engraver beetle, Ips pini (Say). To understand better the hormonal regulation of pheromone biosynthesis in this forest pest, and identify JH III-responsive genes, microarrays were prepared and hybridized to cDNA from midguts of JH III-treated beetles. Expression patterns were confirmed by quantitative real-time RT-PCR. JH III co-ordinately regulated mevalonate pathway genes and many other genes implicated in pheromone biosynthesis. Sex differences in basal levels of mevalonate pathway genes were consistent with their role in male-specific pheromone biosynthesis. This is the first microarray-based study of the developmental and hormonal regulation of insect pheromone biosynthesis.lld:pubmed
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pubmed-article:16640731pubmed:authorpubmed-author:KeelingC ICIlld:pubmed
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pubmed-article:16640731pubmed:pagination207-16lld:pubmed
pubmed-article:16640731pubmed:dateRevised2006-11-15lld:pubmed
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pubmed-article:16640731pubmed:year2006lld:pubmed
pubmed-article:16640731pubmed:articleTitleEffects of juvenile hormone on gene expression in the pheromone-producing midgut of the pine engraver beetle, Ips pini.lld:pubmed
pubmed-article:16640731pubmed:affiliationDepartment of Biochemistry and Molecular Biology, University of Nevada, Reno, USA. ckeeling@mac.comlld:pubmed
pubmed-article:16640731pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:16640731pubmed:publicationTypeResearch Support, U.S. Gov't, Non-P.H.S.lld:pubmed
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