| pubmed-article:16610235 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:16610235 | lifeskim:mentions | umls-concept:C0008059 | lld:lifeskim |
| pubmed-article:16610235 | lifeskim:mentions | umls-concept:C0036536 | lld:lifeskim |
| pubmed-article:16610235 | lifeskim:mentions | umls-concept:C0036537 | lld:lifeskim |
| pubmed-article:16610235 | lifeskim:mentions | umls-concept:C0229671 | lld:lifeskim |
| pubmed-article:16610235 | lifeskim:mentions | umls-concept:C0178602 | lld:lifeskim |
| pubmed-article:16610235 | lifeskim:mentions | umls-concept:C0022885 | lld:lifeskim |
| pubmed-article:16610235 | lifeskim:mentions | umls-concept:C0011546 | lld:lifeskim |
| pubmed-article:16610235 | lifeskim:mentions | umls-concept:C0392366 | lld:lifeskim |
| pubmed-article:16610235 | lifeskim:mentions | umls-concept:C0456603 | lld:lifeskim |
| pubmed-article:16610235 | lifeskim:mentions | umls-concept:C0205464 | lld:lifeskim |
| pubmed-article:16610235 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:16610235 | pubmed:dateCreated | 2006-4-13 | lld:pubmed |
| pubmed-article:16610235 | pubmed:abstractText | Dissociation between GH bioactivity (bio-GH) and GH immunoactivity (immuno-GH) is due to the heterogeneity of the molecule: the measurements do not always provide reliable information on the bio-GH. We studied the ratio of bio-GH and immuno-GH during pharmacological secretion tests in 211 sera to study the concentration-response curve of the assay (C1), 16 samples of normally growing subjects with idiopathic short stature (C2), 13 samples from patients with GH deficiency (GHD1) and 6 samples of 3 patients with GHD and normal provocative tests (GHD2). GH bioactivity was determined by the Nb2 cell proliferation assay (bio-GH) and immuno-GH by a time-resolved immunofluorometric assay (IFMA) (immuno-GH). A non-linear negative relationship between the serum bio-GH/immuno-GH ratio and serum immuno-GH was observed in C1. In log-log plotting representation, two cut-off lines were drawn: a vertical cut-off line separating above-below cut-off serum peak immuno-GH values in provocative tests, and a diagonal cut-off line separating normal-abnormal serum bio-GH/immunoGH ratio; four areas were defined. GHD1 had normal ratios, but below cut-off peak immuno-GH responses. P2 and P3 of Group GHD2 had abnormal ratios in samples with low serum immuno-GH but only P2 had autosomal dominant mutation. P1 had the same autosomal dominant isolated GHD as P2 but a low normal ratio. Our data underline the importance of relatively low serum GH concentrations in mediating GH biological actions. An abnormal serum bio-GH/immuno-GH ratio might explain certain cases of GHD and might be useful in detecting abnormal circulating isoforms of GH in patients with growth failure. | lld:pubmed |
| pubmed-article:16610235 | pubmed:language | eng | lld:pubmed |
| pubmed-article:16610235 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16610235 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:16610235 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16610235 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:16610235 | pubmed:month | Feb | lld:pubmed |
| pubmed-article:16610235 | pubmed:issn | 0391-4097 | lld:pubmed |
| pubmed-article:16610235 | pubmed:author | pubmed-author:MarinoRR | lld:pubmed |
| pubmed-article:16610235 | pubmed:author | pubmed-author:RivarolaM AMA | lld:pubmed |
| pubmed-article:16610235 | pubmed:author | pubmed-author:PaganiSS | lld:pubmed |
| pubmed-article:16610235 | pubmed:author | pubmed-author:BozzolaMM | lld:pubmed |
| pubmed-article:16610235 | pubmed:author | pubmed-author:TauberMM | lld:pubmed |
| pubmed-article:16610235 | pubmed:author | pubmed-author:BelgoroskyAA | lld:pubmed |
| pubmed-article:16610235 | pubmed:author | pubmed-author:MaceirasMM | lld:pubmed |
| pubmed-article:16610235 | pubmed:author | pubmed-author:BerenszteinEE | lld:pubmed |
| pubmed-article:16610235 | pubmed:author | pubmed-author:BozzoliMM | lld:pubmed |
| pubmed-article:16610235 | pubmed:author | pubmed-author:TravaglinoPP | lld:pubmed |
| pubmed-article:16610235 | pubmed:author | pubmed-author:ChalerE AEA | lld:pubmed |
| pubmed-article:16610235 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:16610235 | pubmed:volume | 29 | lld:pubmed |
| pubmed-article:16610235 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:16610235 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:16610235 | pubmed:pagination | 109-14 | lld:pubmed |
| pubmed-article:16610235 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:16610235 | pubmed:meshHeading | pubmed-meshheading:16610235... | lld:pubmed |
| pubmed-article:16610235 | pubmed:meshHeading | pubmed-meshheading:16610235... | lld:pubmed |
| pubmed-article:16610235 | pubmed:meshHeading | pubmed-meshheading:16610235... | lld:pubmed |
| pubmed-article:16610235 | pubmed:meshHeading | pubmed-meshheading:16610235... | lld:pubmed |
| pubmed-article:16610235 | pubmed:meshHeading | pubmed-meshheading:16610235... | lld:pubmed |
| pubmed-article:16610235 | pubmed:meshHeading | pubmed-meshheading:16610235... | lld:pubmed |
| pubmed-article:16610235 | pubmed:meshHeading | pubmed-meshheading:16610235... | lld:pubmed |
| pubmed-article:16610235 | pubmed:meshHeading | pubmed-meshheading:16610235... | lld:pubmed |
| pubmed-article:16610235 | pubmed:meshHeading | pubmed-meshheading:16610235... | lld:pubmed |
| pubmed-article:16610235 | pubmed:meshHeading | pubmed-meshheading:16610235... | lld:pubmed |
| pubmed-article:16610235 | pubmed:meshHeading | pubmed-meshheading:16610235... | lld:pubmed |
| pubmed-article:16610235 | pubmed:meshHeading | pubmed-meshheading:16610235... | lld:pubmed |
| pubmed-article:16610235 | pubmed:meshHeading | pubmed-meshheading:16610235... | lld:pubmed |
| pubmed-article:16610235 | pubmed:meshHeading | pubmed-meshheading:16610235... | lld:pubmed |
| pubmed-article:16610235 | pubmed:meshHeading | pubmed-meshheading:16610235... | lld:pubmed |
| pubmed-article:16610235 | pubmed:year | 2006 | lld:pubmed |
| pubmed-article:16610235 | pubmed:articleTitle | Dose dependency of the serum bio/immuno GH ratio in children during pharmacological secretion tests. | lld:pubmed |
| pubmed-article:16610235 | pubmed:affiliation | Endocrinology Laboratory, Garrahan Pediatric Hospital, Buenos Aires, Argentina. | lld:pubmed |
| pubmed-article:16610235 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:16610235 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
