Linked Life Data

16609691

Source:http://linkedlifedata.com/resource/pubmed/id/16609691

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pubmed-article:16609691pubmed:abstractTextPhospholipid transfer protein (PLTP) is a serum glycoprotein with a central role in high-density lipoprotein metabolism. We created a fusion protein in which enhanced green fluorescent protein (EGFP) was fused to the carboxyl-terminus of PLTP. Stably transfected HepG2 cells, which overexpress this fusion protein, were generated. PLTP-EGFP was translocated into the ER and fluoresced within the biosynthetic pathway, showing a marked concentration in the Golgi complex. The transfected cells secreted into the growth medium phospholipid transfer activity 7-fold higher than that of the mock-transfected controls. The medium of the PLTP-EGFP - expressing cells displayed EGFP fluorescence, demonstrating that both the PLTP and the EGFP moieties had attained a biologically active conformation. However, the specific activity of PLTP-EGFP in the medium was markedly reduced as compared with that of endogenous PLTP. This suggests that the EGFP attached to the carboxyl-terminal tail of PLTP interferes with the interaction of PLTP with its substrates or with the lipid transfer process itself. Fluorescently tagged PLTP is a useful tool for elucidating the intracellular functions of PLTP and the interaction of exogenously added PLTP with cells, and will provide a means of monitoring the distribution of exogenously added PLTP between serum lipoprotein subspecies.lld:pubmed
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pubmed-article:16609691pubmed:pagination117-25lld:pubmed
pubmed-article:16609691pubmed:dateRevised2006-11-15lld:pubmed
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pubmed-article:16609691pubmed:year2006lld:pubmed
pubmed-article:16609691pubmed:articleTitlePlasma phospholipid transfer protein fused with green fluorescent protein is secreted by HepG2 cells and displays phosphatidylcholine transfer activity.lld:pubmed
pubmed-article:16609691pubmed:affiliationDepartment of Molecular Medicine, National Public HealthInstitute, Biomedicum, Helsinki, Finland.lld:pubmed
pubmed-article:16609691pubmed:publicationTypeJournal Articlelld:pubmed
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pubmed-article:16609691pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed
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