| pubmed-article:1660886 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:1660886 | lifeskim:mentions | umls-concept:C0035820 | lld:lifeskim |
| pubmed-article:1660886 | lifeskim:mentions | umls-concept:C0020291 | lld:lifeskim |
| pubmed-article:1660886 | lifeskim:mentions | umls-concept:C0597357 | lld:lifeskim |
| pubmed-article:1660886 | lifeskim:mentions | umls-concept:C0815000 | lld:lifeskim |
| pubmed-article:1660886 | lifeskim:mentions | umls-concept:C0031621 | lld:lifeskim |
| pubmed-article:1660886 | lifeskim:mentions | umls-concept:C0034826 | lld:lifeskim |
| pubmed-article:1660886 | lifeskim:mentions | umls-concept:C0443301 | lld:lifeskim |
| pubmed-article:1660886 | lifeskim:mentions | umls-concept:C1620102 | lld:lifeskim |
| pubmed-article:1660886 | pubmed:issue | 35 | lld:pubmed |
| pubmed-article:1660886 | pubmed:dateCreated | 1992-1-17 | lld:pubmed |
| pubmed-article:1660886 | pubmed:abstractText | The relationship between muscarinic receptor activation of phosphoinositide hydrolysis and the sequestration of cell surface muscarinic receptors has been examined for both intact and digitonin-permeabilized human SK-N-SH neuroblastoma cells. Addition of the aminosteroid 1-[6-[[17 beta-3-methoxyestra-1,3,5(10)-trien-17-yl]amino] hexyl]-1H-pyrrole-2,5-dione (U-73122) to intact cells resulted in the inhibition of oxotremorine-M-stimulated inositol phosphate release and of Ca2+ signaling by greater than 75%. In contrast, when phospholipase C was directly activated by the addition of the calcium ionophore ionomycin, inclusion of U-73122 had little inhibitory effect. Addition of U-73122 to intact cells also inhibited the agonist-induced sequestration of cell surface muscarinic receptors and their subsequent down-regulation with an IC50 value (4.1 microM) similar to that observed for inhibition of inositol phosphate release (3.7 microM). In contrast, when oxotremorine-M-stimulated phosphoinositide hydrolysis was inhibited by depletion of extracellular Ca2+, no reduction in the extent of receptor sequestration was observed. When introduced into digitonin-permeabilized cells, U-73122 more markedly inhibited inositol phosphate release elicited by either oxotremorine-M or guanosine-5'-O-(3-thiotriphosphate) than that induced by added Ca2+. Addition of oxotremorine-M to permeabilized cells resulted in muscarinic receptor sequestration and down-regulation. Both the loss of muscarinic acetylcholine receptors and activation of phosphoinositide hydrolysis in permeabilized cells were inhibited by the inclusion of guanosine-5'-O-(2-thiodiphosphate). The results indicate that the agonist-induced sequestration of muscarinic acetylcholine receptor in SK-N-SH cells requires the involvement of a GTP-binding protein but not the production of phosphoinositide-derived second messenger molecules. | lld:pubmed |
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| pubmed-article:1660886 | pubmed:language | eng | lld:pubmed |
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| pubmed-article:1660886 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:1660886 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:1660886 | pubmed:month | Dec | lld:pubmed |
| pubmed-article:1660886 | pubmed:issn | 0021-9258 | lld:pubmed |
| pubmed-article:1660886 | pubmed:author | pubmed-author:FisherS KSK | lld:pubmed |
| pubmed-article:1660886 | pubmed:author | pubmed-author:BleasdaleJ... | lld:pubmed |
| pubmed-article:1660886 | pubmed:author | pubmed-author:ThompsonA KAK | lld:pubmed |
| pubmed-article:1660886 | pubmed:author | pubmed-author:MostafapourS... | lld:pubmed |
| pubmed-article:1660886 | pubmed:author | pubmed-author:DenlingerL... | lld:pubmed |
| pubmed-article:1660886 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:1660886 | pubmed:day | 15 | lld:pubmed |
| pubmed-article:1660886 | pubmed:volume | 266 | lld:pubmed |
| pubmed-article:1660886 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:1660886 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:1660886 | pubmed:pagination | 23856-62 | lld:pubmed |
| pubmed-article:1660886 | pubmed:dateRevised | 2011-11-17 | lld:pubmed |
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| pubmed-article:1660886 | pubmed:year | 1991 | lld:pubmed |
| pubmed-article:1660886 | pubmed:articleTitle | The aminosteroid U-73122 inhibits muscarinic receptor sequestration and phosphoinositide hydrolysis in SK-N-SH neuroblastoma cells. A role for Gp in receptor compartmentation. | lld:pubmed |
| pubmed-article:1660886 | pubmed:affiliation | Neuroscience Laboratory, University of Michigan, Ann Arbor 48104-1687. | lld:pubmed |
| pubmed-article:1660886 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:1660886 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
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