Linked Life Data

1660595

Source:http://linkedlifedata.com/resource/pubmed/id/1660595

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pubmed-article:1660595pubmed:abstractTextThe mechanism of regulation of intracellular pH (pHi) in dispersed acini from the rat mandibular salivary gland has been studied with a microfluorimetric imaging method and the pH probe 2',7'-bis(2-carboxyethyl)-5(and -6)-carboxyfluorescein. The pHi in the TRIS/HEPES-buffered standard solution was 7.29 +/- 0.01. Addition of 1 mumol/l acetylcholine (ACh) or ionomycin caused a sustained increase in the pHi. These agents decreased pHi in the absence of external Na+ or in the presence of amiloride. The rate of pHi recovery from an acid load after NH+4 prepulse was a linear function of pHi and increased as pHi became more acidic. Addition of ACh shifted the relationship towards a more alkaline pHi range. The increase in pHi induced by ACh or ionomycin was not inhibited by the protein kinase C inhibitors staurosporine (10 nM) and 1-(5-isoquinolinesulfonyl)-1-methylpiperazine (50 mumol/l). Addition of 0.1-1 mumol/l phorbol 12-myristate 13-acetate (TPA) had little effect on pHi within 10 min; however, exposure to TPA for 120 min resulted in a significant rise in pHi. In Ca(2+)-free solution with 50 mumol/l 8-(diethylamino)-octyl-3,4,5-trimethoxybenzoate, the ACh-induced rise in both pHi and cytosolic Ca2+ concentration was suppressed. ACh and ionomycin caused an increment of amiloride-sensitive acid output into the extracellular fluid, while 20 mumol/l 1-oleoyl-2-acetylglycerol had little effect on it. It was concluded that (a) stimulation with ACh activated the Na+/H+ antiport in the plasma membrane, (b) ACh also stimulated the intracellular acid production but acid extrusion by the Na+/H+ antiport prevented the cell from intracellular acidification, and (c) the major route of signal transduction for the ACh-induced activation of the Na+/H+ antiport was independent of protein kinase C but was dependent on the rise in cytosolic Ca2+ concentration. The implication of the cytosolic acidification and cell volume change in pHi regulation is discussed.lld:pubmed
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pubmed-article:1660595pubmed:dateRevised2007-11-15lld:pubmed
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pubmed-article:1660595pubmed:articleTitleMicrofluorimetric imaging study of the mechanism of activation of the Na+/H+ antiport by muscarinic agonist in rat mandibular acinar cells.lld:pubmed
pubmed-article:1660595pubmed:affiliationDepartment of Physiology, Tohoku University School of Medicine, Sendai, Japan.lld:pubmed
pubmed-article:1660595pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:1660595pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed
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