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pubmed-article:1654915pubmed:abstractTextThe configurations of immunoglobulin, T-cell receptor beta chain and bcl-2 genes were analyzed in lympho-proliferative disorders using nonradioactive digoxigenin-labeled probes. The studies demonstrated the reliability of digoxigenin-labeled probes for the detection of single-copy genes after Southern blotting of genomic DNA: 1 microgram and sometimes even 0.2 microgram of restriction endonuclease-digested DNA could be detected either by JH, C beta or bcl-2 probes. The intensity of the signal was consistently satisfactory, and there was no background problem. Control experiments showed neither false-positive nor false-negative results caused by the use of the nonradioactive detection system.lld:pubmed
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pubmed-article:1654915pubmed:dateRevised2007-11-14lld:pubmed
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pubmed-article:1654915pubmed:articleTitleGene rearrangements detected by nonradioactive digoxigenin-labeled DNA probes.lld:pubmed
pubmed-article:1654915pubmed:affiliationDepartment of Genetics and Pathology, Medical Academy, Szczecin, Poland.lld:pubmed
pubmed-article:1654915pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:1654915pubmed:publicationTypeResearch Support, U.S. Gov't, P.H.S.lld:pubmed