| pubmed-article:16543722 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:16543722 | lifeskim:mentions | umls-concept:C0010453 | lld:lifeskim |
| pubmed-article:16543722 | lifeskim:mentions | umls-concept:C0009325 | lld:lifeskim |
| pubmed-article:16543722 | lifeskim:mentions | umls-concept:C1328818 | lld:lifeskim |
| pubmed-article:16543722 | lifeskim:mentions | umls-concept:C0017725 | lld:lifeskim |
| pubmed-article:16543722 | lifeskim:mentions | umls-concept:C0441889 | lld:lifeskim |
| pubmed-article:16543722 | lifeskim:mentions | umls-concept:C0033268 | lld:lifeskim |
| pubmed-article:16543722 | lifeskim:mentions | umls-concept:C1879688 | lld:lifeskim |
| pubmed-article:16543722 | pubmed:issue | 1-2 | lld:pubmed |
| pubmed-article:16543722 | pubmed:dateCreated | 2006-3-17 | lld:pubmed |
| pubmed-article:16543722 | pubmed:abstractText | Recent evidences have demonstrated an important role for glomerular visceral epithelial cell (podocyte) in the development and progression of diabetic nephropathy. We investigated the high-glucose (HG)-triggered signaling pathway and its role in matrix metalloproteinase (MMP) production in murine podocytes. The activity of 92-kDa (MMP-9) gelatinase, but not of 72 kDa (MMP-2), in an HG medium significantly increased during incubation of 2 to 3 days and decreased during incubation of more than 5 days revealed by Gelatin zymography. Opposite to the increases in MMP-9 activity, HG medium produced significant decreases in the protein levels of alpha5(IV) collagen. Changes in MMP-9 activity were associated with the same pattern as MMP-9 mRNA levels in podocytes exposed to HG media. HG medium rapidly activated ERK1/2 MAPK in podocytes. Moreover, ERK1/2 activation was required for HG-induced enhancement of MMP-9 activity and a decrease in the level of alpha5(IV) collagen. HG incubation rapidly induced an increase in the nuclear accumulation of Ets-1 protein. Blocking the ERK pathway suppressed HG-induced expression and nuclear accumulation of transcriptional factor Ets-1, and MMP-9 mRNA expression. We suggest that short- or long-term exposure to HG concentrations increases or decreases MMP-9 production and alpha5(IV) collagen expression in podocytes, this may contribute to the GBM abnormality caused by an imbalance in extracellular matrix (ECM) synthesis and degradation, and may play a critical role in the pathogenesis of proteinuria in diabetic nephropathy. | lld:pubmed |
| pubmed-article:16543722 | pubmed:language | eng | lld:pubmed |
| pubmed-article:16543722 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16543722 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:16543722 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16543722 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:16543722 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16543722 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16543722 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16543722 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16543722 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:16543722 | pubmed:issn | 1015-8987 | lld:pubmed |
| pubmed-article:16543722 | pubmed:author | pubmed-author:WangHaiyanH | lld:pubmed |
| pubmed-article:16543722 | pubmed:author | pubmed-author:LiJingziJ | lld:pubmed |
| pubmed-article:16543722 | pubmed:author | pubmed-author:LiYongqiangY | lld:pubmed |
| pubmed-article:16543722 | pubmed:author | pubmed-author:HuangHaichang... | lld:pubmed |
| pubmed-article:16543722 | pubmed:author | pubmed-author:LiuSenyanS | lld:pubmed |
| pubmed-article:16543722 | pubmed:author | pubmed-author:WangLizhuL | lld:pubmed |
| pubmed-article:16543722 | pubmed:author | pubmed-author:BaiYalingY | lld:pubmed |
| pubmed-article:16543722 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:16543722 | pubmed:volume | 17 | lld:pubmed |
| pubmed-article:16543722 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:16543722 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:16543722 | pubmed:pagination | 57-68 | lld:pubmed |
| pubmed-article:16543722 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:16543722 | pubmed:meshHeading | pubmed-meshheading:16543722... | lld:pubmed |
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| pubmed-article:16543722 | pubmed:meshHeading | pubmed-meshheading:16543722... | lld:pubmed |
| pubmed-article:16543722 | pubmed:meshHeading | pubmed-meshheading:16543722... | lld:pubmed |
| pubmed-article:16543722 | pubmed:meshHeading | pubmed-meshheading:16543722... | lld:pubmed |
| pubmed-article:16543722 | pubmed:meshHeading | pubmed-meshheading:16543722... | lld:pubmed |
| pubmed-article:16543722 | pubmed:meshHeading | pubmed-meshheading:16543722... | lld:pubmed |
| pubmed-article:16543722 | pubmed:meshHeading | pubmed-meshheading:16543722... | lld:pubmed |
| pubmed-article:16543722 | pubmed:meshHeading | pubmed-meshheading:16543722... | lld:pubmed |
| pubmed-article:16543722 | pubmed:meshHeading | pubmed-meshheading:16543722... | lld:pubmed |
| pubmed-article:16543722 | pubmed:meshHeading | pubmed-meshheading:16543722... | lld:pubmed |
| pubmed-article:16543722 | pubmed:meshHeading | pubmed-meshheading:16543722... | lld:pubmed |
| pubmed-article:16543722 | pubmed:meshHeading | pubmed-meshheading:16543722... | lld:pubmed |
| pubmed-article:16543722 | pubmed:year | 2006 | lld:pubmed |
| pubmed-article:16543722 | pubmed:articleTitle | High ambient glucose levels modulates the production of MMP-9 and alpha5(IV) collagen by cultured podocytes. | lld:pubmed |
| pubmed-article:16543722 | pubmed:affiliation | Division of Nephrology, Peking University First Hospital, Beijing, China. | lld:pubmed |
| pubmed-article:16543722 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:16543722 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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