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pubmed-article:1647121pubmed:abstractTextA co-culture system was established between human midgut carcinoid tumour cells and rat fetal cholinergic neurons. In monocultures in serum-free media, only tumour cells survived, while neurons deteriorated. In serum-free co-cultures, neurons displayed outgrowth of neuritic processes. Neurons of neuronal serum-free monocultures thrived if supplemented with conditioned media from tumour cell cultures grown serum-free. This indicates that tumour cells produce transferable growth factor(s) with potent neuronotrophic actions. Immunocytochemical studies indicate that this growth factor resembles nerve growth factor immunologically, since tumour cells were strongly immunoreactive after incubation with a rabbit anti-nerve growth factor antiserum, and furthermore expressed immunoreactive nerve growth factor receptors.lld:pubmed
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pubmed-article:1647121pubmed:articleTitleProduction of transferable neuronotrophic factor(s) by human midgut carcinoid tumour cells; studies using cultures of rat fetal cholinergic neurons.lld:pubmed
pubmed-article:1647121pubmed:affiliationInstitute of Neurobiology, NRCG, University of Gothenburg, Sweden.lld:pubmed
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pubmed-article:1647121pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed
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