| pubmed-article:16416099 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:16416099 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:16416099 | lifeskim:mentions | umls-concept:C0070455 | lld:lifeskim |
| pubmed-article:16416099 | lifeskim:mentions | umls-concept:C1524119 | lld:lifeskim |
| pubmed-article:16416099 | lifeskim:mentions | umls-concept:C0005516 | lld:lifeskim |
| pubmed-article:16416099 | lifeskim:mentions | umls-concept:C0332157 | lld:lifeskim |
| pubmed-article:16416099 | lifeskim:mentions | umls-concept:C0870883 | lld:lifeskim |
| pubmed-article:16416099 | lifeskim:mentions | umls-concept:C0020980 | lld:lifeskim |
| pubmed-article:16416099 | lifeskim:mentions | umls-concept:C0597329 | lld:lifeskim |
| pubmed-article:16416099 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:16416099 | pubmed:dateCreated | 2006-2-14 | lld:pubmed |
| pubmed-article:16416099 | pubmed:abstractText | Permethrin is the most popular synthetic pyrethroid insecticide used in agriculture and public health. For the assessment of human exposure to permethrin, a competitive indirect enzyme-linked immunosorbent assay (ELISA) for the detection of the glycine conjugate of a major metabolite, cis-/trans-3-(2,2-dichlorovinyl)-2,2-dimethylcyclopropane-1-carboxylic acid (DCCA), of permethrin was developed based on a polyclonal antibody. An assay based on an antibody with a high sensitivity was optimized and characterized. The IC50 value and the detection range for trans-DCCA-glycine, in the assay buffer were 1.2 and 0.2-7.0 microg/L, respectively. The antibody recognized trans-DCCA-glycine and the mixture of cis-/trans-DCCA-glycine with an isomer range from 30:70 to 50:50 nearly equally. Little or no cross-reactivity to permethrin and its other free metabolites or glycine conjugates was measured. The integration of the ELISA and solid-phase extraction which was used to reduce the matrix effect from human urine samples provided for analysis of total cis-/trans-DCCA-glycine at low parts per billion levels in the samples. The limit of quantitation of the target analyte was 1.0 microg/L in urine with a limit of detection of 0.1 microg/L in buffer. This assay might be a useful tool for monitoring human exposure to permethrin. | lld:pubmed |
| pubmed-article:16416099 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16416099 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16416099 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16416099 | pubmed:language | eng | lld:pubmed |
| pubmed-article:16416099 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16416099 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:16416099 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16416099 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16416099 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16416099 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16416099 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16416099 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:16416099 | pubmed:month | Feb | lld:pubmed |
| pubmed-article:16416099 | pubmed:issn | 1618-2642 | lld:pubmed |
| pubmed-article:16416099 | pubmed:author | pubmed-author:HammockBruce... | lld:pubmed |
| pubmed-article:16416099 | pubmed:author | pubmed-author:MaSeung-JinSJ | lld:pubmed |
| pubmed-article:16416099 | pubmed:author | pubmed-author:AhnKi ChangKC | lld:pubmed |
| pubmed-article:16416099 | pubmed:author | pubmed-author:GeeShirley... | lld:pubmed |
| pubmed-article:16416099 | pubmed:author | pubmed-author:TsaiHsing-JuH... | lld:pubmed |
| pubmed-article:16416099 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:16416099 | pubmed:volume | 384 | lld:pubmed |
| pubmed-article:16416099 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:16416099 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:16416099 | pubmed:pagination | 713-22 | lld:pubmed |
| pubmed-article:16416099 | pubmed:dateRevised | 2007-12-3 | lld:pubmed |
| pubmed-article:16416099 | pubmed:meshHeading | pubmed-meshheading:16416099... | lld:pubmed |
| pubmed-article:16416099 | pubmed:meshHeading | pubmed-meshheading:16416099... | lld:pubmed |
| pubmed-article:16416099 | pubmed:meshHeading | pubmed-meshheading:16416099... | lld:pubmed |
| pubmed-article:16416099 | pubmed:meshHeading | pubmed-meshheading:16416099... | lld:pubmed |
| pubmed-article:16416099 | pubmed:meshHeading | pubmed-meshheading:16416099... | lld:pubmed |
| pubmed-article:16416099 | pubmed:meshHeading | pubmed-meshheading:16416099... | lld:pubmed |
| pubmed-article:16416099 | pubmed:meshHeading | pubmed-meshheading:16416099... | lld:pubmed |
| pubmed-article:16416099 | pubmed:meshHeading | pubmed-meshheading:16416099... | lld:pubmed |
| pubmed-article:16416099 | pubmed:year | 2006 | lld:pubmed |
| pubmed-article:16416099 | pubmed:articleTitle | An immunoassay for a urinary metabolite as a biomarker of human exposure to the pyrethroid insecticide permethrin. | lld:pubmed |
| pubmed-article:16416099 | pubmed:affiliation | Department of Entomology and UCD Cancer Research Center, University of California, Davis, CA 95616, USA. | lld:pubmed |
| pubmed-article:16416099 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:16416099 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |
| pubmed-article:16416099 | pubmed:publicationType | Research Support, N.I.H., Extramural | lld:pubmed |
