| pubmed-article:1629327 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:1629327 | lifeskim:mentions | umls-concept:C0038170 | lld:lifeskim |
| pubmed-article:1629327 | lifeskim:mentions | umls-concept:C1511790 | lld:lifeskim |
| pubmed-article:1629327 | lifeskim:mentions | umls-concept:C0032520 | lld:lifeskim |
| pubmed-article:1629327 | lifeskim:mentions | umls-concept:C1514468 | lld:lifeskim |
| pubmed-article:1629327 | lifeskim:mentions | umls-concept:C1954468 | lld:lifeskim |
| pubmed-article:1629327 | lifeskim:mentions | umls-concept:C1955827 | lld:lifeskim |
| pubmed-article:1629327 | lifeskim:mentions | umls-concept:C0439831 | lld:lifeskim |
| pubmed-article:1629327 | pubmed:issue | 7 | lld:pubmed |
| pubmed-article:1629327 | pubmed:dateCreated | 1992-8-20 | lld:pubmed |
| pubmed-article:1629327 | pubmed:abstractText | In order to identify methicillin-resistant staphylococci from clinical sources with ease and reliability, enzymatic detection of polymerase chain reaction (ED-PCR) was applied. ED-PCR is based on the capture of amplified products via biotin-streptavidin affinity and the detection of an incorporated hapten in amplified products with an enzyme-linked antibody. In order to identify methicillin-resistant staphylococci of all species, a 150-bp fragment of the mecA gene was targeted for ED-PCR. After PCR was performed with a pair of biotin and dinitrophenol 5'-labeled primers, the reaction mixture was applied to a microtiter well precoated with streptavidin. Thereafter, bound PCR products were detected colorimetrically with alkaline phosphatase-conjugated anti-dinitrophenol antibody. The extraction of DNA from staphylococcal cells for PCR was simplified so that it could be performed within one tube. The total assay, including PCR, took less than 3 h. The sensitivity of mecA gene detection ranged from greater than 5 x 10(2) CFU per tube for Staphylococcus aureus to greater than 5 x 10(3) CFU per tube for Staphylococcus epidermidis. Genotyping results obtained by ED-PCR of 161 tested strains from the colonies (97 strains of S. aureus and 64 strains of coagulase-negative staphylococci) were compared with the phenotypic susceptibilities of the strains to oxacillin. The results of ED-PCR showed excellent agreement with the MICs of oxacillin with very few exceptions; only one strain of S. aureus and two strains of coagulase-negative staphylococci were found to possess the mecA gene, which was discrepant with their phenotypes. Fifty-five blood culture samples were also tested by ED-PCR. For staphylococcal isolates in 33 of the cultures, oxacillin MICs were >4 microgram/ml; 31 of the 33 staphylococcal isolates were determined by ED-PCR to be mecA gene positive. These results suggest that ED-PCR can be used with reasonable confidence in the clinical microbiological laboratory. | lld:pubmed |
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| pubmed-article:1629327 | pubmed:language | eng | lld:pubmed |
| pubmed-article:1629327 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1629327 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:1629327 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:1629327 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:1629327 | pubmed:month | Jul | lld:pubmed |
| pubmed-article:1629327 | pubmed:issn | 0095-1137 | lld:pubmed |
| pubmed-article:1629327 | pubmed:author | pubmed-author:SugiuraMM | lld:pubmed |
| pubmed-article:1629327 | pubmed:author | pubmed-author:KonnoMM | lld:pubmed |
| pubmed-article:1629327 | pubmed:author | pubmed-author:KawakamiSS | lld:pubmed |
| pubmed-article:1629327 | pubmed:author | pubmed-author:YamaneAA | lld:pubmed |
| pubmed-article:1629327 | pubmed:author | pubmed-author:NittaAA | lld:pubmed |
| pubmed-article:1629327 | pubmed:author | pubmed-author:UbukataKK | lld:pubmed |
| pubmed-article:1629327 | pubmed:author | pubmed-author:NakagamiSS | lld:pubmed |
| pubmed-article:1629327 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:1629327 | pubmed:volume | 30 | lld:pubmed |
| pubmed-article:1629327 | pubmed:geneSymbol | mecA | lld:pubmed |
| pubmed-article:1629327 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:1629327 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:1629327 | pubmed:pagination | 1728-33 | lld:pubmed |
| pubmed-article:1629327 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:1629327 | pubmed:year | 1992 | lld:pubmed |
| pubmed-article:1629327 | pubmed:articleTitle | Rapid detection of the mecA gene in methicillin-resistant staphylococci by enzymatic detection of polymerase chain reaction products. | lld:pubmed |
| pubmed-article:1629327 | pubmed:affiliation | Department of Clinical Pathology, School of Medicine, Teikyo University, Tokyo, Japan. | lld:pubmed |
| pubmed-article:1629327 | pubmed:publicationType | Journal Article | lld:pubmed |
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