| pubmed-article:16232222 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:16232222 | lifeskim:mentions | umls-concept:C0008059 | lld:lifeskim |
| pubmed-article:16232222 | lifeskim:mentions | umls-concept:C0332307 | lld:lifeskim |
| pubmed-article:16232222 | lifeskim:mentions | umls-concept:C0011854 | lld:lifeskim |
| pubmed-article:16232222 | lifeskim:mentions | umls-concept:C0011306 | lld:lifeskim |
| pubmed-article:16232222 | lifeskim:mentions | umls-concept:C0031437 | lld:lifeskim |
| pubmed-article:16232222 | lifeskim:mentions | umls-concept:C0542341 | lld:lifeskim |
| pubmed-article:16232222 | lifeskim:mentions | umls-concept:C0205349 | lld:lifeskim |
| pubmed-article:16232222 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:16232222 | pubmed:dateCreated | 2005-10-19 | lld:pubmed |
| pubmed-article:16232222 | pubmed:abstractText | The importance of dendritic cells (DC) in the activation of T cells and in the maintenance of self-tolerance is well known. We investigated whether alterations in phenotype and function of DC may contribute to the pathogenesis of Type 1 diabetes (T1DM). Mature DC (mDC) from 18 children with T1DM and 10 age-matched healthy children were tested. mDC, derived from peripheral blood monocytes cultured for 6 days in presence of interleukin (IL)-4 and granulocyte-macrophage colony stimulating factor (GM-CSF) and stimulated with lipopolysaccharide (LPS) for the last 24 h, were phenotyped for the expression of the co-stimulatory molecules B7.1 and B7.2. In six patients and six controls allogenic mixed leucocyte reaction (AMLR) was performed using mDC and cord blood-derived naive T cells at a DC/T naive ratio of 1 : 200. Proliferation was assessed on day 7 by [(3)H]-thymidine incorporation assay. Mature DC derived from patients showed, compared with controls, a reduced expression of B7.1 [mean of fluorescence intensity (MFI): 36.2 +/- 14.3 versus 72.9 +/- 34.5; P = 0.004] and B7.2 (MFI: 122.7 +/- 67.5 versus 259.6 +/- 154.1; P = 0.02). We did not find differences in the HLA-DR expression (P = 0.07). Moreover, proliferative response of allogenic naive T cells cultured with mDC was impaired in the patients (13471 +/- 9917.2 versus 40976 +/- 24527.2 cpm, P = 0.04). We also measured IL-10 and IL-12 concentration in the supernatant of DC cultures. Interestingly, we observed in the patients a sevenfold higher level of IL-10 (P = 0.07) and a ninefold lower level of IL-12 (P = 0.01). Our data show a defect in the expression of the co-stimulatory molecules and an impairment of DC priming function, events that might contribute to T1DM pathogenesis. | lld:pubmed |
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| pubmed-article:16232222 | pubmed:language | eng | lld:pubmed |
| pubmed-article:16232222 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16232222 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:16232222 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:16232222 | pubmed:month | Nov | lld:pubmed |
| pubmed-article:16232222 | pubmed:issn | 0009-9104 | lld:pubmed |
| pubmed-article:16232222 | pubmed:author | pubmed-author:AngeliniFF | lld:pubmed |
| pubmed-article:16232222 | pubmed:author | pubmed-author:RossiPP | lld:pubmed |
| pubmed-article:16232222 | pubmed:author | pubmed-author:PiccininiSS | lld:pubmed |
| pubmed-article:16232222 | pubmed:author | pubmed-author:Manca... | lld:pubmed |
| pubmed-article:16232222 | pubmed:author | pubmed-author:Del DucaEE | lld:pubmed |
| pubmed-article:16232222 | pubmed:author | pubmed-author:PaccianiVV | lld:pubmed |
| pubmed-article:16232222 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:16232222 | pubmed:volume | 142 | lld:pubmed |
| pubmed-article:16232222 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:16232222 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:16232222 | pubmed:pagination | 341-6 | lld:pubmed |
| pubmed-article:16232222 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:16232222 | pubmed:meshHeading | pubmed-meshheading:16232222... | lld:pubmed |
| pubmed-article:16232222 | pubmed:year | 2005 | lld:pubmed |
| pubmed-article:16232222 | pubmed:articleTitle | Altered phenotype and function of dendritic cells in children with type 1 diabetes. | lld:pubmed |
| pubmed-article:16232222 | pubmed:affiliation | Department of Pediatrics, Tor Vergata University, Rome, Italy. angelini@med.uniroma2.it | lld:pubmed |
| pubmed-article:16232222 | pubmed:publicationType | Journal Article | lld:pubmed |
| entrez-gene:56822 | entrezgene:pubmed | pubmed-article:16232222 | lld:entrezgene |
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