| pubmed-article:1622287 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:1622287 | lifeskim:mentions | umls-concept:C0042153 | lld:lifeskim |
| pubmed-article:1622287 | lifeskim:mentions | umls-concept:C0021267 | lld:lifeskim |
| pubmed-article:1622287 | lifeskim:mentions | umls-concept:C0014834 | lld:lifeskim |
| pubmed-article:1622287 | lifeskim:mentions | umls-concept:C0038636 | lld:lifeskim |
| pubmed-article:1622287 | lifeskim:mentions | umls-concept:C0814810 | lld:lifeskim |
| pubmed-article:1622287 | lifeskim:mentions | umls-concept:C0185125 | lld:lifeskim |
| pubmed-article:1622287 | lifeskim:mentions | umls-concept:C1706701 | lld:lifeskim |
| pubmed-article:1622287 | lifeskim:mentions | umls-concept:C0243072 | lld:lifeskim |
| pubmed-article:1622287 | pubmed:issue | 6 | lld:pubmed |
| pubmed-article:1622287 | pubmed:dateCreated | 1992-8-6 | lld:pubmed |
| pubmed-article:1622287 | pubmed:abstractText | An Escherichia coli strain, B-62, that was isolated from a clinical source and was epidemiologically unrelated to E. coli K-12 was the source of chromosomal DNA for a sucrose utilization system (Scr+) in the construction of a plasmid, pST621. The cloned insert of a gene encoding Scr+ in pST621 conferred a sucrose-positive phenotype onto transformed cells of E. coli K-12 derivatives. Sucrase activity of the transformants was as high as that which would correspond to a "gene dosage effect" of a vector plasmid pBR322, whereas the transformants' sucrose uptake activity was always lower than that of E. coli B-62. A region within an XhoI-SacI fragment (3.2 kb) of pBR322-glyA was replaced in the construction of another plasmid, pST5R7, by a fragment (about 2.6 kb) of pST622 containing the gene encoding Scr+. A genetically stable Scr+ derivative of E. coli K-12 was obtained by introducing the gene encoding Scr+ onto E. coli chromosome via homologous recombination between pST5R7 and the chromosome and subsequent plasmid segregation. The use of low-copy-number plasmid RP4 as a cloning vector was also effective for enhancing the stability of Scr+. Tryptophan producers E. coli SGIII1032S, in which the gene encoding Scr+ was cloned onto the chromosome, and E. coli SGIII1032, which carried Scr+ plasmid RP4.5R7, produced from 6% sucrose in shake flasks (33 degrees C, 96 h) 2.3 and 5.7 g of tryptophan per liter, respectively. | lld:pubmed |
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| pubmed-article:1622287 | pubmed:language | eng | lld:pubmed |
| pubmed-article:1622287 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1622287 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:1622287 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:1622287 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:1622287 | pubmed:month | Jun | lld:pubmed |
| pubmed-article:1622287 | pubmed:issn | 0099-2240 | lld:pubmed |
| pubmed-article:1622287 | pubmed:author | pubmed-author:OkabeMM | lld:pubmed |
| pubmed-article:1622287 | pubmed:author | pubmed-author:OkamotoRR | lld:pubmed |
| pubmed-article:1622287 | pubmed:author | pubmed-author:AibaSS | lld:pubmed |
| pubmed-article:1622287 | pubmed:author | pubmed-author:TsunekawaHH | lld:pubmed |
| pubmed-article:1622287 | pubmed:author | pubmed-author:AzumaSS | lld:pubmed |
| pubmed-article:1622287 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:1622287 | pubmed:volume | 58 | lld:pubmed |
| pubmed-article:1622287 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:1622287 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:1622287 | pubmed:pagination | 2081-8 | lld:pubmed |
| pubmed-article:1622287 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:1622287 | pubmed:meshHeading | pubmed-meshheading:1622287-... | lld:pubmed |
| pubmed-article:1622287 | pubmed:year | 1992 | lld:pubmed |
| pubmed-article:1622287 | pubmed:articleTitle | Acquisition of a sucrose utilization system in Escherichia coli K-12 derivatives and its application to industry. | lld:pubmed |
| pubmed-article:1622287 | pubmed:affiliation | Mercian Co., Central Research Laboratories, Kanagawa, Japan. | lld:pubmed |
| pubmed-article:1622287 | pubmed:publicationType | Journal Article | lld:pubmed |
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