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pubmed-article:16220906pubmed:abstractTextA new class of small nonfluorescent fluorogenic substrates becomes brightly fluorescent after beta-lactamase hydrolysis with up to 153-fold enhancement in the fluorescence intensity. Less than 500 fM of beta-lactamase in cell lysates can be readily detected, and beta-lactamase expression in living cells can be imaged with a red fluorescence derivative. These new fluorogenic substrates should find uses in clinical diagnostics and facilitate the applications of beta-lactamase as a biosensor.lld:pubmed
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pubmed-article:16220906pubmed:articleTitleNovel fluorogenic substrates for imaging beta-lactamase gene expression.lld:pubmed
pubmed-article:16220906pubmed:affiliationDepartment of Molecular and Medical Pharmacology, Crump Institute for Molecular Imaging, University of California, Los Angeles, California 90095-1770, USA.lld:pubmed
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