| pubmed-article:1620590 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:1620590 | lifeskim:mentions | umls-concept:C0085356 | lld:lifeskim |
| pubmed-article:1620590 | lifeskim:mentions | umls-concept:C0330390 | lld:lifeskim |
| pubmed-article:1620590 | lifeskim:mentions | umls-concept:C0017337 | lld:lifeskim |
| pubmed-article:1620590 | lifeskim:mentions | umls-concept:C0003940 | lld:lifeskim |
| pubmed-article:1620590 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:1620590 | lifeskim:mentions | umls-concept:C0086860 | lld:lifeskim |
| pubmed-article:1620590 | lifeskim:mentions | umls-concept:C2911684 | lld:lifeskim |
| pubmed-article:1620590 | lifeskim:mentions | umls-concept:C0185117 | lld:lifeskim |
| pubmed-article:1620590 | pubmed:issue | 12 | lld:pubmed |
| pubmed-article:1620590 | pubmed:dateCreated | 1992-8-5 | lld:pubmed |
| pubmed-article:1620590 | pubmed:abstractText | Arylsulfatase, produced by Chlamydomonas reinhardtii during sulfur-limited growth, is secreted into the periplasmic space and is readily assayed using a chromogenic substrate. To assess the usefulness of the gene encoding arylsulfatase (ars) as a reporter gene in C. reinhardtii, we have fused the promoter region of the beta 2-tubulin gene (tubB2) to the coding region of an ars genomic clone to form a tubB2/ars chimeric sequence. This construct was introduced into C. reinhardtii, strain CC425 (cw-15, arg-2), via cotransformation with the argininosuccinate lyase gene (which complements the arg-2 lesion) (1). Transformants expressing arylsulfatase (Ars) in sulfur-sufficient medium were isolated and subsequently shown to contain the tubB2/ars gene. RNA analysis determined that tubB2/ars transcripts accumulated in these cells. Abundance of the chimeric transcript increased immediately following deflagellation in a manner similar to that of the endogenous tubB2 transcript. Thus, chimeric genes incorporating ars coding sequences and heterologous promoters can be used to examine regulated gene expression in C. reinhardtii. | lld:pubmed |
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| pubmed-article:1620590 | pubmed:language | eng | lld:pubmed |
| pubmed-article:1620590 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1620590 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:1620590 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:1620590 | pubmed:month | Jun | lld:pubmed |
| pubmed-article:1620590 | pubmed:issn | 0305-1048 | lld:pubmed |
| pubmed-article:1620590 | pubmed:author | pubmed-author:GrossmanA RAR | lld:pubmed |
| pubmed-article:1620590 | pubmed:author | pubmed-author:WeeksD PDP | lld:pubmed |
| pubmed-article:1620590 | pubmed:author | pubmed-author:DaviesJ PJP | lld:pubmed |
| pubmed-article:1620590 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:1620590 | pubmed:day | 25 | lld:pubmed |
| pubmed-article:1620590 | pubmed:volume | 20 | lld:pubmed |
| pubmed-article:1620590 | pubmed:geneSymbol | tubB2 | lld:pubmed |
| pubmed-article:1620590 | pubmed:geneSymbol | ars | lld:pubmed |
| pubmed-article:1620590 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:1620590 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:1620590 | pubmed:pagination | 2959-65 | lld:pubmed |
| pubmed-article:1620590 | pubmed:dateRevised | 2010-9-9 | lld:pubmed |
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| pubmed-article:1620590 | pubmed:year | 1992 | lld:pubmed |
| pubmed-article:1620590 | pubmed:articleTitle | Expression of the arylsulfatase gene from the beta 2-tubulin promoter in Chlamydomonas reinhardtii. | lld:pubmed |
| pubmed-article:1620590 | pubmed:affiliation | Carnegie Institution of Washington, Department of Plant Biology, Stanford, CA 94305. | lld:pubmed |
| pubmed-article:1620590 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:1620590 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |
| pubmed-article:1620590 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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